Commonly Used Reagents


Publication Name:  Current Protocols in Microbiology
Unit Number:  Appendix 2A
DOI:  10.1002/9780471729259.mca02as00
Online Posting Date:  July, 2005
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Abstract

    Recipes

  • Acid, concentrated stock solutions

  • Acid precipitation solution

  • Alsever's solution

  • Ammonium hydroxide, concentrated stock solution

  • Ammonium acetate, 10 M

  • Ammonium sulfate, saturated

  • ATP, 100 mM

  • Base, concentrated stock solutions

  • BBS (BES‐buffered solution), 2×

  • BCIP, 5% (w/v)

  • BSA (bovine serum albumin), 10% (w/v)

  • CaCl2, 1 M

  • Carbonate buffer

  • Denhardt solution, 100×

  • DEPC (diethylpyrocarbonate)‐treated solutions

  • DNase I, RNase‐free (1 mg/ml)

  • dNTPs: dATP, dTTP, dCTP, and dGTP

  • DPBS (Dulbecco's phosphate‐buffered saline)

  • DTT (dithiothreitol), 1 M

  • EDTA (ethylenediaminetetraacetic acid), 0.5 M (pH 8.0)

  • Ethidium bromide staining solution

  • FBS (fetal bovine serum)

  • FBS, heat inactivated

  • Formamide loading buffer, 2×

  • HBSS (Hanks' balanced salt solution)

  • HCl, 1 M

  • HeBS (HEPES‐buffered saline) solution, 2×

  • KCl, 1 M

  • 2‐ME (2‐mercaptoethanol), 50 mM

  • MgCl2, 1 M

  • MgSO4, 1 M

  • MOPS buffer

  • NaCl, 5 M

  • NaOH, 10 M

  • PCR amplification buffer, 10×

  • Phenol, buffered

  • Phenol/chloroform/isoamyl alcohol, 25:24:1 (v/v/v)

  • Phosphate‐buffered saline (PBS)

  • Phosphate‐buffered saline containing potassium (KPBS)

  • PMSF (phenylmethylsulfonyl fluoride), 100 mM

  • Potassium acetate buffer, 0.1 M

  • Potassium phosphate buffer, 0.1 M

  • RNase A stock solution, DNase‐free, 2 mg/ml

  • Saline, 0.9%

  • Saponin, 10% (w/v)

  • SDS, 20% (w/v)

  • SDS sample buffer

  • SED (standard enzyme diluent)

  • Sodium acetate, 3 M

  • Sodium acetate buffer, 0.1 M

  • Sodium phosphate buffer, 0.1 M

  • Silanized glassware

  • SSC (sodium chloride/sodium citrate), 20×

  • SSPE (sodium chloride/sodium phosphate/EDTA), 20×

  • T4 DNA ligase buffer, 10×

  • TAE buffer, 50×

  • TBE (Tris/borate/EDTA) buffer, 10×

  • TBS (Tris‐buffered saline)

  • TCA (trichloroacetic acid), 100% (w/v)

  • TE (Tris/EDTA) buffer

  • TEA (triethanolamine) solution

  • Tris⋅Cl, 1 M

  • Triton X‐100, 10% (w/v)

  • TTBS (Tween 20/TBS)

  • Urea loading buffer, 2×

     
 
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Table of Contents

  • Literature Cited
  • Tables
     
 
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Materials

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Figures

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Literature Cited

Literature Cited
   Chemical Rubber Company (CRC). 1975. CRC Handbook of Biochemistry and Molecular Biology, Physical and Chemical Data, 3rd ed., Vol. 1. CRC Press, Boca Raton, Fla.
   Mohan, C. (ed.). 1997. Buffers: A Guide for the Preparation and Use of Buffers in Biological Systems. Calbiochem, San Diego, Calif.
   Wallace, R.B. and Miyada, C.G. 1987. Oligonucleotide probes for the screening of recombinant DNA libraries. In Methods of Enzymology, Vol. 152: Guide to Molecular Cloning Techniques (S.L. Berger and A.R. Kimmel, eds.) pp. 432‐442. Academic Press, San Diego.
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