Preliminary Staining of Bacteria: Simple Stains

Rita B. Moyes1, Jackie Reynolds2, Donald P. Breakwell3

1 Texas A&M University, College Station, Texas, 2 Richland College, Dallas, Texas, 3 Brigham Young University, Provo, Utah
Publication Name:  Current Protocols in Microbiology
Unit Number:  Appendix 3E
DOI:  10.1002/9780471729259.mca03es15
Online Posting Date:  November, 2009
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Abstract

Simple staining involves directly staining the bacterial cell with a positively charged dye in order to see bacterial detail, in contrast to negative staining where the bacteria remain unstained against a dark background. Curr. Protoc. Microbiol. 15:A.3E.1‐A.3E.5. © 2009 by John Wiley & Sons, Inc.

Keywords: simple stain; bacteria; methylene blue; crystal violet; Ziehl's carbolfuschin

     
 
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Table of Contents

  • Basic Protocol 1: Bacterial Simple Stain
  • Reagents and Solutions
  • Commentary
  • Literature Cited
  • Figures
  • Tables
     
 
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Materials

Basic Protocol 1: Bacterial Simple Stain

  Materials
  • Bacterial broth culture or colony
  • Dyes‐methylene blue, crystal violet, Ziehl's carbol fuschin (see reciperecipes)
  • Immersion Oil
  • Microscope slides (cleaned with 95% ethanol followed by water rinse and dried with laboratory wipes)
  • Inoculating loop
  • Clothespin or clamp
  • Bunsen burner
  • Staining rack
  • Bibulous paper or paper towels
  • Microscope
  • Biohazard container
  • Additional reagents and equipment for observing the slide using a compound light microscope (unit 2.1)
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Figures

Videos

Literature Cited

   Willey, J.M., Sherwood, L.M., and Woolverton, C.J. 2008. Microbiology, 7th Edition (L. Prescott, J.P. Harley, and D.A. Klein, eds.) Chapters 2 and 3. McGraw Hill, New York.
Internet Resources
  http://www.microbelibrary.org
  Select the visual collection icon and search the free image bank for your desired organism and stain.
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