Aseptic Technique

Tomasz Bykowski1, Brian Stevenson2

1 Center for Medical Education, Warsaw, Poland, 2 Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine, Lexington, Kentucky
Publication Name:  Current Protocols in Microbiology
Unit Number:  Appendix 4D
DOI:  10.1002/9780471729259.mca04ds11
Online Posting Date:  November, 2008
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Abstract

This chapter describes common laboratory procedures that can reduce the risk of culture contaminations (sepsis), collectively referred as “aseptic technique.” Two major strategies of aseptic work are described: using a Bunsen burner and a laminar flow hood. Both methods are presented in the form of general protocols applicable to a variety of laboratory tasks such as pipetting and dispensing aliquots, preparing growth media, and inoculating, passaging, and spreading microorganisms on petri dishes. Curr. Protoc. Microbiol. 11:A.4D.1‐A.4D.11. © 2008 by John Wiley & Sons, Inc.

Keywords: aseptic technique; sterilization; bacteriology; Bunsen burner; laminar flow; laminar hood; virology; cell culture

     
 
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Table of Contents

  • Introduction
  • Strategic Planning
  • Safety Considerations
  • Techniques for Maintaining Aseptic Conditions
  • Troubleshooting
  • Figures
  • Tables
     
 
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Materials

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Figures

Videos

Literature Cited

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