Anaerobic Cell Culture

Allison M. Speers1, Dena L. Cologgi1, Gemma Reguera1

1 Michigan State University, East Lansing, Michigan
Publication Name:  Current Protocols in Microbiology
Unit Number:  Appendix 4F
DOI:  10.1002/9780471729259.mca04fs12
Online Posting Date:  February, 2009
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Although the ability of some microorganisms to grow without O2 has long been recognized, the application of new methodologies has greatly expanded the known diversity and potential of anaerobic microorganisms and processes. In particular, anaerobic techniques that permit the successful cultivation of microorganisms on solid media have opened new avenues for the study of the physiology and metabolic potential of many new microorganisms using molecular, genomic, and proteomic tools. One technique above all has proven instrumental for anaerobic studies over the years: the use of the anaerobic chamber. This unit gives a brief description of the methods used for the cultivation of anaerobic microorganisms, and describes in detail the principles and applications of anaerobic chambers, with special emphasis on vinyl glove boxes. The methodologies described in this unit should provide the interested but inexperienced investigator with the basic tools to successfully cultivate anaerobic microorganisms and study anaerobic processes. Curr. Protoc. Microbiol. 12:A.4F.1‐A.4F.16. © 2009 by John Wiley & Sons, Inc.

Keywords: anaerobes; gassing station; anaerobic chamber; anaerobic techniques; glove box

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Table of Contents

  • Introduction
  • Overview and Principles
  • Anaerobic Chambers: Operation and Maintenance
  • Preparing Anaerobic Liquid Medium
  • Preparing Anaerobic Solid Medium
  • Cultivation of Hydrogen Sulfide–Producing Microorganisms
  • Acknowledgements
  • Literature Cited
  • Figures
  • Tables
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Literature Cited

Literature Cited
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   Nottingham, P.M. and Hungate, R.E. 1969. Methanogenic fermentation of benzoate. J. Bacteriol. 98:1170‐1172.
   Pouliquen, F., Blanc, C., Arretz, E., Labat, I., Tournier‐Lasserve, J., Ladousse, A., Nougayrede, J., Savin, G., Ivaldi, R., Nicolas, M., Fialaire, J., Millischer, R., Azema, C., Espagno, L., Hemmer, H., and Perrot, J. 2005. Hydrogen sulfide. Ullman's Encyclopedia of Industrial Chemistry, 7th ed. Wiley‐VCH, Weinheim, Germany.
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