Growth and Maintenance of Quail Fibrosarcoma QM5 Cells

Anh Tran1, Alicia Berard1, Kevin M. Coombs1

1 University of Manitoba and Manitoba Centre for Proteomics and Systems Biology, Winnipeg, Manitoba, Canada
Publication Name:  Current Protocols in Microbiology
Unit Number:  Appendix 4G
DOI:  10.1002/9780471729259.mca04gs14
Online Posting Date:  August, 2009
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Abstract

This appendix describes techniques for culturing quail fibrosarcoma QM5 cell lines, one of the preferred cell lines in which many avian reovirus studies take place. Curr. Protoc. Microbiol. 14:A.4G.1‐A.4G.7. © 2009 by John Wiley & Sons, Inc.

Keywords: avian cell line; cell culture; quail cell culture

     
 
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Table of Contents

  • Introduction
  • Basic Protocol 1: Growth and Maintenance of QM5 Cell Line
  • Basic Protocol 2: Plating QM5 Cells
  • Basic Protocol 3: Freezing and Thawing QM5 Cell Line
  • Reagents and Solutions
  • Commentary
  • Literature Cited
  • Figures
  • Tables
     
 
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Materials

Basic Protocol 1: Growth and Maintenance of QM5 Cell Line

  Materials
  • Confluent QM5 cells in a 150‐cm2 flask
  • 1× PBS/EDTA (see recipe)
  • 1× trypsin/EDTA (see recipe)
  • Complete 1× M199, prewarmed to 37°C (see recipe)
  • 15‐ml conical tube, sterile
  • Additional reagents and equipment for counting cells using a hemacytometer ( appendix 4A)

Basic Protocol 2: Plating QM5 Cells

  Materials
  • Trypsinized QM5 cells (see protocol 1)
  • Complete 1× M199 growth medium, prewarmed to 37°C (see recipe)
  • Appropriate‐sized vessels (depending on use)

Basic Protocol 3: Freezing and Thawing QM5 Cell Line

  Materials
  • Confluent QM5 cells in a 150‐cm2 flask
  • 1× PBS/EDTA (see recipe)
  • 1× trypsin/EDTA (see recipe)
  • Complete 1× M199, prewarmed to 37°C (see recipe)
  • 100% fetal bovine serum (FBS), prechilled at 4°C
  • Freezing medium (see recipe)
  • Liquid nitrogen
  • 15‐ml conical tube, sterile
  • Low‐speed benchtop centrifuge
  • Cryogenic vials
  • −80°C freezer
  • 37°C water bath
  • 75‐cm2 culture flask
  • Additional reagents and equipment for counting cells using a hemacytometer ( appendix 4A)
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Figures

Videos

Literature Cited

Literature Cited
   Antin, P.B. and C.P. Ordahl, C.P. 1991. Isolation and characterization of an avian myogenic cell line. Dev. Biol. 143:111‐121.
   Moscovici, C., Moscovici, M.G., Jimenez, H., Lai, M.M., Hayman, M.J., and Vogt, P.K. 1977. Continuous tissue culture cell lines derived from chemically induced tumors of Japanese quail. Cell 11:95‐103.
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