Growth and Maintenance of Baby Hamster Kidney (BHK) Cells

Raquel Hernandez1, Dennis T. Brown1

1 North Carolina State University, Raleigh, North Carolina
Publication Name:  Current Protocols in Microbiology
Unit Number:  Appendix 4H
DOI:  10.1002/9780471729259.mca04hs17
Online Posting Date:  May, 2010
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Abstract

The BHK21 cell line was established in 1961 from the kidneys of 5 Syrian hamsters from litter number 21. Since this time, this cell line has been a laboratory standard for the growth of countless viruses and the study of many biological processes. The specific use for the growth of Sindbis virus is described, although it may apply to many types of viruses. Curr. Protoc. Microbiol. 17:A.4H.1‐A.4H.7. © 2010 by John Wiley & Sons, Inc.

Keywords: BHK; vertebrate cells; cell culture

     
 
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Table of Contents

  • Introduction
  • Basic Protocol 1: Propagation of BHK Cells
  • Basic Protocol 2: Freezing and Thawing Stocks of BHK Cultures
  • Reagents and Solutions
  • Commentary
  • Literature Cited
     
 
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Materials

Basic Protocol 1: Propagation of BHK Cells

  Materials
  • Baby hamster kidney (BHK) cells (ATCC #CCL‐10), growing in culture (if starting from frozen stock, see protocol 2)
  • 1× PBS‐D (see recipe)
  • 0.25% trypsin (see recipe)
  • Versene solution (see recipe)
  • 1× complete EMEM medium (see recipe)
  • 25‐ or 75‐cm2 tissue culture flasks
  • 5‐ or 10‐ml pipets
  • Conical tubes
  • Sterile bottles

Basic Protocol 2: Freezing and Thawing Stocks of BHK Cultures

  Materials
  • BHK cells (see protocol 1)
  • 0.25% trypsin (see recipe)
  • Versene solution (see recipe)
  • 1× PBS‐D (see recipe)
  • 1× complete EMEM medium (see recipe)
  • Freezing medium (see recipe) made with EMEM
  • 2‐ml cryotubes
  • Nalgene Cryo 1°C freezing container
  • Liquid nitrogen freezer
  • 37°C water bath
  • 25‐cm2 flask
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Figures

Videos

Literature Cited

Literature Cited
   Stoker, M. and Macpherson, I. 1964. Syrian hamster fibroblast cell line Bhk21 and its derivatives. Nature 203:1355–1357.
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