Growth and Maintenance of Chick Embryo Fibroblasts (CEF)
Primary cultures of chick embryo fibroblasts (CEF) are widely used for the cultivation of viruses. Protocols for the growth and maintenance of CEF cells in the laboratory are provided. Curr. Protoc. Microbiol. 17:A.4I.1‐A.4I.8. © 2010 by John Wiley & Sons, Inc.
Keywords: chick embryo fibroblasts; primary cell lines; cell culture
Basic Protocol 1: Preparation of Primary Chick Embryo Fibroblast (CEF) Culture
Basic Protocol 2: Freezing and Thawing of Chick Embryo Fibroblast (CEF) Cultures