Design and Generation of Gene‐Targeting Vectors

Benedikt Wefers1, Wolfgang Wurst2, Ralf Kühn3

1 German Research Center for Environmental Health, Munich, Germany, 2 Deutsches Zentrum für Neurodegenerative Erkrankungen e.V. (DZNE), Munich, Germany, 3 Technical University Munich, Munich, Germany
Publication Name:  Current Protocols in Mouse Biology
Unit Number:   
DOI:  10.1002/9780470942390.mo100179
Online Posting Date:  March, 2011
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Abstract

This unit provides an overview of the major types of mutant alleles that can be generated by gene targeting in ES cells. It presents the growing public resources of premade gene targeting vectors, modified ES cells, and mutant mice. General guidelines for the design of targeting vectors are followed by protocols for the construction of vectors to generate knockout (KO), conditional KO, and subtle mutant alleles. Curr. Protoc. Mouse Biol. 1:199‐211. © 2011 by John Wiley & Sons, Inc.

Keywords: gene targeting; conditional KO mice; targeting vector design; knockout mice; EUCOMM/KOMP

     
 
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Table of Contents

  • Introduction
  • Protocols for the Construction of Gene Targeting Vectors
  • Concluding Remarks
  • Literature Cited
  • Figures
     
 
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Materials

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Literature Cited

Literature Cited
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