Overview of the Measurement of Lipids and Lipoproteins in Mice

Anne Tailleux1, Bart Staels1

1 Institut Pasteur de Lille, Lille, France
Publication Name:  Current Protocols in Mouse Biology
Unit Number:   
DOI:  10.1002/9780470942390.mo110001
Online Posting Date:  June, 2011
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Abstract

Experimental mouse models are widely used for preclinical research on dyslipidemia, atherosclerosis, and cardiometabolic diseases. This unit reports the most commonly used biochemical analysis methods available to determine the lipid and lipoprotein phenotype in the mouse. The discussed methods include: the qualitative and quantitative analysis of lipids, apolipoproteins, and lipoproteins (with a specific emphasis on species‐specificities of mice and humans), and the activity assay of major enzymes involved in lipoprotein remodeling (LCAT, PLTP, and LPL). The unit also discusses the most frequently used functional tests to analyze lipid/lipoprotein metabolism in vivo, including triglyceride metabolism, reverse cholesterol transport, intestinal lipid absorption, and secretion. Curr. Protoc. Mouse Biol. 1:265‐277 © 2011 by John Wiley & Sons, Inc.

Keywords: lipids; lipoproteins; apolipoproteins; mouse

     
 
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Table of Contents

  • Introduction
  • Quantitative and Qualitative Analysis of Circulating Lipids and Lipoproteins
  • Measurement of Enzyme Activities
  • Functional Tests In Vivo
  • Conclusion: Technologies on the Horizon
  • Literature Cited
  • Figures
  • Tables
     
 
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Materials

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Figures

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Literature Cited

Literature Cited
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