PET, SPECT, CT, and MRI in Mouse Cardiac Phenotyping: An Overview

André Constantinesco1, Philippe Choquet1, Christian Goetz1, Laurent Monassier2

1 Laboratoire d'Imagerie Préclinique, Service de Biophysique et Médecine Nucléaire, Hôpitaux Universitaires de Strasbourg, Strasbourg, France, 2 Laboratoire de Neurobiologie et Pharmacologie Cardiovasculaire, Université de Strasbourg, Strasbourg, France
Publication Name:  Current Protocols in Mouse Biology
Unit Number:   
DOI:  10.1002/9780470942390.mo110225
Online Posting Date:  June, 2012
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Abstract

This overview first summarizes the last decade of continuous developments and improvements in pre‐clinical imaging methods that are now essential tools for in vivo evaluation of cardiac morphology and function in living mice, involving nuclear emission of labeled molecules (micro‐PET and micro‐SPECT) and electromagnetic wave interactions with biological tissues (micro‐CT and micro‐MRI). In the following, and for better understanding, the basic physical principles and specific technical innovations of the aforementioned imaging methods are reviewed. Specificity, sensitivity, and spatial and temporal resolutions, together with the corresponding advantages and weaknesses of each method are then discussed, and cardiac image‐acquisition protocols and illustrative examples are given for each modality. Emerging hybrid cardiac imaging is also presented and illustrated. Then, recent biological insights provided by mouse cardiac imaging are presented. Finally, imaging strategies in mouse cardiac phenotyping involving the aforementioned methods, adding metabolic and molecular information to morphological data, are emphasized and discussed. Curr. Protoc. Mouse Biol. 2:129‐144 © 2012 by John Wiley & Sons, Inc.

Keywords: pre‐clinical imaging; phenotyping; mouse models; cardiology; PET; SPECT; CT; MRI

     
 
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Table of Contents

  • 1: Introduction: A Short Historical Perspective on In Vivo Microimaging of Mouse Cardiac Morphology and Function Using PET, SPECT, CT, and MRI
  • 2: Pre‐Clinical Imaging Background Physics and Examples of Mouse Cardiac Imaging Protocols and Results
  • 3: Cardiac Hybrid Imaging in the Mouse
  • 4: New Biological Insights Provided by Mouse Cardiac Imaging
  • Conclusion
  • Literature Cited
  • Figures
     
 
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Materials

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Literature Cited

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