General Considerations for Mouse Survival Surgery

Bonnie L. Lyons1, Linda L. Waterman1

1 The Jackson Laboratory, Bar Harbor, Maine
Publication Name:  Current Protocols in Mouse Biology
Unit Number:   
DOI:  10.1002/9780470942390.mo120121
Online Posting Date:  September, 2012
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Abstract

Personnel performing surgery on mice commonly lack formal medical training and most training in mouse surgery focuses on the mechanical skills required for a procedure with less emphasis on perioperative techniques. Consequently, a basic concept that underpins successful surgery, aseptic technique, may not be fully understood or incorporated into the training process. This unit provides a framework in which to plan and carry out surgical procedures in mice using appropriate aseptic technique. Curr. Protoc. Mouse Biol. 2:263‐271 © 2012 by John Wiley & Sons, Inc.

Keywords: surgery; aseptic; mouse

     
 
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Table of Contents

  • Introduction
  • Aseptic Surgical Technique
  • Presurgical Preparation
  • Intraoperative Procedures
  • Postoperative Recovery
  • Conclusion
  • Literature Cited
  • Figures
  • Tables
     
 
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Materials

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Figures

Videos

Literature Cited

   AWA. 2010. Animal Welfare Act. United States Department of Agriculture (USDA), National Agricultural Library.
   Bradfield, J.F., Schachtman, T.R., McLaughlin, R.M., and Steffen, E.K. 1992. Behavioral and physiologic effects of inapparent wound infection in rats. Lab. Anim. Sci. 42:572‐578.
   Clough, G. 1982. Environmental effects on animals used in biomedical research. Biol. Rev. Camb. Philos. Soc. 57:487‐523.
   Committee for the Update of the Guide for the Care and Use of Laboratory Animals, Division on Earth and Life Studies. 2011. Guide for the Care and Use of Laboratory Animals, Eighth ed. The National Academies Press, Washington, D.C.
   Foreman, O., Kavirayani, A.M., Griffey, S.M., Reader, R., and Shultz, L.D. 2011. Opportunistic bacterial infections in breeding colonies of the NSG mouse strain. Vet. Pathol. 48:495‐499.
   Hostler, D., Zhou, J., Tortorici, M.A., Bies, R.R., Rittenberger, J.C., Empey, P.E., Kochanek, P.M., Callaway, C.W., and Poloyac, S.M. 2010. Mild hypothermia alters midazolam pharmacokinetics in normal healthy volunteers. Drug Metab. Dispos. 38:781‐788.
   The Jackson Laboratory, 2012. Workshop on Surgical Techniques in the Laboratory Mouse. Bar Harbor, ME: The Jackson Laboratory.
   Keen, J.N., Austin, M., Huang, L.S., Messing, S., and Wyatt, J.D. 2010. Efficacy of soaking in 70% isopropyl alcohol on aerobic bacterial decontamination of surgical instruments and gloves for serial mouse laparotomies. J. Am. Assoc. Lab. Anim. Sci. 49:832‐837.
   PHS. 2002. Public Health Service Policy on Humane Care and Use of Laboratory Animals, Office of Laboratory Animal Welfare: Public Health Service Policy on Humane Care and Use of Laboratory Animals. Bethesda, Md.
   Raabe, B.M., Artwohl, J.E., Purcell, J.E., Lovaglio, J., and Fortman, J.D. 2011. Effects of weekly blood collection in C57BL/6 mice. J. Am. Assoc. Lab. Anim. Sci. 50:680‐685.
   Tortorici, M.A., Kochanek, P.M., and Poloyac, S.M. 2007. Effects of hypothermia on drug disposition, metabolism, and response: A focus of hypothermia‐mediated alterations on the cytochrome P450 enzyme system. Crit. Care Med. 35:2196‐2204.
Internet Resources
   http://courses.jax.org/event‐listings.html
  The Jackson Laboratory's Web site for the Workshop on Surgical Techniques in the Laboratory Mouse.
   http://awic.nal.usda.gov/government‐and‐professional‐resources/federal‐laws/animal‐welfare‐act
  Animal Welfare Act. United States Department of Agriculture (USDA), National Agricultural Library.
   http://grants.nih.gov/grants/olaw/references/phspol.htm
  Public Health Service Policy on Humane Care and Use of Laboratory Animals, Office of Laboratory Animal Welfare: Public Health Service Policy on Humane Care and Use of Laboratory Animals.
   http://www.nap.edu/catalog.php?record_id=12910
  Committee for the Update of the Guide for the Care and Use of Laboratory Animals, Division on Earth and Life Studies. 2011. Guide for the Care and Use of Laboratory Animals, Eighth Edition.
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