Acute Asthma Models to Ovalbumin in the Mouse

François Daubeuf1, Nelly Frossard1

1 Laboratoire d'Innovation Thérapeutique, Faculté de Pharmacie, UMR7200 CNRS/Université de Strasbourg, Illkirch Cedex, France
Publication Name:  Current Protocols in Mouse Biology
Unit Number:   
DOI:  10.1002/9780470942390.mo120202
Online Posting Date:  March, 2013
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Abstract

Human asthma is defined as a chronic inflammatory disease of the airways. Animal models are required to study asthma pathophysiology and identify and/or evaluate new therapeutic strategies. Several models of asthma have been developed in mice to mimic asthma symptoms, and can be divided in two groups as acute and chronic models. They are characterized by airway hyperresponsiveness (AHR), inflammation, and remodeling. Several experimental procedures have been implemented, the one mostly used being acute asthma models to ovalbumin. It comprises a sensitization step in the presence of aluminum hydroxide as an adjuvant, and a second step where mice are challenged with the allergen introduced directly into the airways to induce the modeled asthma features. This article describes procedures to efficiently and reproducibly obtain acute asthma features in mice, with ovalbumin as the allergen, which allow group comparisons and/or assessment of the activity of drug candidates. Curr. Protoc. Mouse Biol. 3:31‐37 © 2013 by John Wiley & Sons, Inc.

Keywords: asthma; allergy; inflammation; bronchial hyperresponsiveness

     
 
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Table of Contents

  • Introduction
  • Basic Protocol 1: Acute Ovalbumin Asthma Models
  • Support Protocol 1: Models of Asthma Induced by Ovalbumin
  • Commentary
  • Literature Cited
  • Figures
  • Tables
     
 
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Materials

Basic Protocol 1: Acute Ovalbumin Asthma Models

  Materials
  • Ovalbumin grade V (Sigma‐Aldrich, cat. no. A5503)
  • Sterile saline
  • Aluminum hydroxide (Sigma‐Aldrich, cat. no. 23918‐6)
  • 9‐week‐old male BALB/c or C57BL/6 mice
  • Anesthetics (50 mg/kg Ketamine/3 mg/kg Xylazine), stored at 4°C
  • Precision balance (0.1 mg)
  • 50‐ml Falcon tubes
  • 1.5‐ml microtubes, sterile
  • 5 ml sterile culture tubes
  • 15‐ml sterile centrifuge tubes
  • Rotator mixer
  • 1‐ml sterile syringes
  • 25‐G needles
  • Vortex mixer
  • Sterile tips
  • Precision pipets (20 µl and 1000 µl)
  • Heating blanket or heating lamp
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Figures

Videos

Literature Cited

Literature Cited
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