Acquiring, Recording, and Analyzing Pathology Data from Experimental Mice: An Overview

Cheryl L. Scudamore1

1 Mary Lyon Centre, MRC Harwell, Harwell Science and Innovation Campus, Oxfordshire
Publication Name:  Current Protocols in Mouse Biology
Unit Number:   
DOI:  10.1002/9780470942390.mo130200
Online Posting Date:  March, 2014
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Abstract

Pathology is often underutilized as an end point in mouse studies in academic research because of a lack of experience and expertise. The use of traditional pathology techniques including necropsy and microscopic analysis can be useful in identifying the basic processes underlying a phenotype and facilitating comparison with equivalent human diseases. This overview aims to provide a guide and reference to the acquisition, recording, and analysis of high‐quality pathology data from experimental mice in an academic research setting. Curr. Protoc. Mouse Biol. 4:1‐10. © 2014 by John Wiley & Sons, Inc.

Keywords: mouse; pathology; analysis; quantitation; histology

     
 
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Table of Contents

  • Introduction
  • Acquiring High‐Quality Pathology Data
  • Recording Pathology Data
  • Analysis of Pathology Data
  • Summary
  • Literature Cited
  • Figures
  • Tables
     
 
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Materials

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Figures

Videos

Literature Cited

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Internet Resources
  http://www.toxpath.org/inhand.asp
  InHAND guides Web site. Guide to harmonized nomenclature for laboratory animal pathology being updated by the pharmaceutical industry.
  http://search.oecd.org/officialdocuments/displaydocumentpdf/?cote=ENV/JM/MONO(2011)47&doclanguage=en
  Accessed 15 Nov 2013. OECD guidance document, which explains protocols and sample sizes required for large‐scale rodent studies to detect tumor formation in response to toxicants that could be used as a guide for sample size decisions with studies of oncogenicity.
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