Protection of 2′‐Hydroxy Functions of Ribonucleosides

Colin B. Reese1

1 King's College London, London, United Kingdom
Publication Name:  Current Protocols in Nucleic Acid Chemistry
Unit Number:  Unit 2.2
DOI:  10.1002/0471142700.nc0202s00
Online Posting Date:  May, 2001
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The main purpose of this article is to discuss 2′‐protection in the context of effective oligoribonucleotide synthesis. Emphasis is placed on the 2′‐protecting groups of choice in the synthesis of oligo‐and polyribonucleotides, and the requirements that a protective group must satisfy to become the 2′‐hydroxyl‐protecting group of choice. Finally, the unit discusses the issue of 2′‐O‐acyl and 2′‐O‐silyl group migration to the 3′‐hydroxy function of ribonucleosides during protection, along with the consequences of the conditions used for their removal on the stability of internucleotide linkages.

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Table of Contents

  • Considerations for 2′‐Protecting Groups in Oligoribonucleotide Synthesis
  • Protection of the 2′‐Hydroxy Function in Oligoribonucleotide Synthesis
  • Acknowledgment
  • Figures
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