Protection of 5′‐Hydroxy Functions of Nucleosides

H. Seliger1

1 University of Ulm, Ulm, Germany
Publication Name:  Current Protocols in Nucleic Acid Chemistry
Unit Number:  Unit 2.3
DOI:  10.1002/0471142700.nc0203s00
Online Posting Date:  May, 2001
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Abstract

The 5‐hydroxy group is the primary hydroxy group of nucleosides. It is mandatory to protect 5‐hydroxyls in all methods of oligonucleotide synthesis that require nucleoside synthons. This unit discusses a wide variety of acid‐labile and base‐labile protecting groups, as well as enzymatic methods for 5‐protection and deprotection.

     
 
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Table of Contents

  • Scope of this Overview
  • Acid‐Labile Protecting Groups
  • Blocking Groups Labile to Nonacidic Conditions
  • Enzymatic Methods for 5′‐Hydroxyl Protection and Deprotection
  • Protection of 5′‐Hydroxy Functions: Remaining Problems, Considerations, and Options
  • Literature Cited
  • Figures
  • Tables
     
 
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Materials

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Literature Cited

Literature Cited
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