Modification of the 5′ Terminus of Oligonucleotides for Attachment of Reporter and Conjugate Groups

Nguyen T. Thuong1, Ulysse Asseline1

1 Centre de Biophysique Moléculaire, CNRS, Orléans, France
Publication Name:  Current Protocols in Nucleic Acid Chemistry
Unit Number:  Unit 4.2
DOI:  10.1002/0471142700.nc0402s00
Online Posting Date:  May, 2001
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Abstract

Reporter and conjugate groups can be added directly to the 5′ terminus of oligonucleotides by appropriate modification. Conjugate groups can be used to increase the affinity of complementary strands, induce irreversible modification of target sequences, or enable sequences to recognize and permeate target cell membranes. This overview discusses the 5′ modifications that can be used and strategies for the covalent attachment of ligands to the modified oligonucleotides. Step‐by‐step protocols for attachment of conjugate groups are given elsewhere in the series.

     
 
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Table of Contents

  • Synthesis
  • Characterization
  • Properties
  • Conclusion
  • Literature Cited
  • Figures
     
 
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Materials

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Figures

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Literature Cited

Literature Cited
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