Attachment of Reporter and Conjugate Groups to the 3′ Termini of Oligonucleotides

Marc M. Greenberg1

1 Colorado State University, Fort Collins, Colorado
Publication Name:  Current Protocols in Nucleic Acid Chemistry
Unit Number:  Unit 4.5
DOI:  10.1002/0471142700.nc0405s02
Online Posting Date:  May, 2001
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Conjugation of oligonucleotides at the 3 terminus is less common because this site is used for covalent linkage to solid‐phase oligonucleotide synthesis supports. However, 3‐oligonucleotide conjugates have several valuable physicochemical properties, including their ability to stabilize nucleic acid hybridization complexes and to retard the activity of exonucleases. This unit discusses methods for preparing oligonucleotides conjugated at the 3 terminus.

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Table of Contents

  • Modified Solid Phase Synthesis Supports and Phosphoramidites
  • Solid Phase Synthesis Supports for Producing 3′‐Fuctional Groups Suitable for Conjugation
  • Postsynthetic Conjugation
  • Summary
  • Literature Cited
  • Figures
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Literature Cited

Literature Cited
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