Engineering Terminal Disulfide Bonds Into DNA

Gary D. Glick1

1 University of Michigan, Ann Arbor, Michigan
Publication Name:  Current Protocols in Nucleic Acid Chemistry
Unit Number:  Unit 5.7
DOI:  10.1002/0471142700.nc0507s13
Online Posting Date:  August, 2003
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Abstract

This protocol presents a simple and general means of modifying nucleic acids with disulfide cross‐links. These cross‐links serve as powerful tools to probe the structure, dynamics, thermodynamics, folding, and function of DNA and RNA, much in the way that cystine cross‐links have been used to study proteins. The chemistry described has been used to synthesize disulfide‐cross‐linked hairpins and duplexes, higher‐order structures such as triplexes, non‐ground‐state conformations, and tRNAs. since the cross‐links form quantitatively by mild air oxidation and do not purturb either secondary or tertiary structure, this modification should prove quite useful for the study of nucleic acids.

Keywords: nucleic acid; disulfide cross‐link; structure; dynamics; stability

     
 
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Table of Contents

  • Design of an Ideal Cross‐Link
  • Disulfide Cross‐Linked DNA Duplexes
  • Disulfide Cross‐Linked DNA Triplexes
  • Applications
  • Synthesis of Disulfide Cross‐Linked DNAs Using Convertible Nucleosides
  • Acknowledgements
  • Literature Cited
  • Figures
  • Tables
     
 
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Materials

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Literature Cited

Literature Cited
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