NMR Determination of Oligonucleotide Structure

Thomas L. James1

1 University of California, San Francisco, California
Publication Name:  Current Protocols in Nucleic Acid Chemistry
Unit Number:  Unit 7.2
DOI:  10.1002/0471142700.nc0702s00
Online Posting Date:  May, 2001
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This unit provides an overview of the use of NMR to determine oligonucleotide structure. It covers basic NMR spectral properties, acquisition of interproton distance restraints and torsion angle restraints, structure refinement, assessment of the quality of the structure obtained. Software programs used in the process are also described.

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Table of Contents

  • Overview of Oligonucleotide Structure Determination by NMR
  • Basic NMR Spectral Parameters
  • Acquisition of Interproton Distance Restraints
  • Aquisition of Torsion‐Angle Restraints
  • Structure Refinement
  • Assessment of Structure Quality
  • Analysis of Nucleic Acid Structure
  • Acknowledgments
  • Literature Cited
  • Figures
  • Tables
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Literature Cited

Literature Cited
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