Recent Advances in RNA Structure Determination by NMR

M. Hennig1, J.R. Williamson1, A.S. Brodsky2, J.L. Battiste3

1 The Scripps Research Institute, La Jolla, California, 2 Dana‐Farber Cancer Institute, Boston, Massachusetts, 3 Harvard Medical School, Boston, Massachusetts
Publication Name:  Current Protocols in Nucleic Acid Chemistry
Unit Number:  Unit 7.7
DOI:  10.1002/0471142700.nc0707s02
Online Posting Date:  May, 2001
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Abstract

Despite recent advances in the solution of NMR structures of RNA and RNA‐ligand complexes, the rate limiting step remains the gathering of a large number of NOE and torsion restraints. Additional sources of information for structure determination of larger RNA molecules have recently become available, and it is possible to supplement NOE and J‐coupling data with the measurement of dipolar couplings and cross‐correlated relaxation rates in high‐resolution NMR spectroscopy.

     
 
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Table of Contents

  • Isotopic Labeling of RNA
  • Assignment of RNA NMR Resonances
  • NOE Measurement
  • Hydrogen Bonds in RNA NMR Structures
  • Measurement of Backbone Torsions
  • Novel Parameters for Liquid‐State NMR Structure Determination
  • RNA Structures
  • Prospects for Large RNA Molecules
  • Figures
  • Tables
     
 
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Materials

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Literature Cited

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