Deformability Calculation for Estimation of the Relative Stability of Chemically Modified RNA Duplexes

Yoshiaki Masaki1, Mitsuo Sekine1, Kohji Seio1

1 Department of Life Science and Technology, Tokyo Institute of Technology, Yokohama
Publication Name:  Current Protocols in Nucleic Acid Chemistry
Unit Number:  Unit 7.27
DOI:  10.1002/cpnc.25
Online Posting Date:  March, 2017
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Chemical modification of RNA duplexes alters their stability. We have attempted to develop a computational approach to estimate the thermal stability of chemically modified duplexes. These studies revealed that the deformability of chemically modified RNA duplexes, calculated from molecular dynamics simulations, could be used as a good indicator for estimating the effect of chemical modification on duplex thermal stability. This unit describes how deformability calculation can be applied to estimate the relative stability of chemically modified RNA duplexes. © 2017 by John Wiley & Sons, Inc.

Keywords: deformability; nucleic acid; duplex thermal stability; chemical modification; RNA

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Table of Contents

  • Commentary
  • Literature Cited
  • Figures
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Literature Cited

Literature Cited
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