Key Experimental Approaches in DNA Nanotechnology

Nadrian C. Seeman1

1 New York University, New York, New York
Publication Name:  Current Protocols in Nucleic Acid Chemistry
Unit Number:  Unit 12.1
DOI:  10.1002/0471142700.nc1201s09
Online Posting Date:  August, 2002
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DNA nanotechnology combines unusual DNA motifs with sticky‐ended cohesion to build polyhedral objects, topological targets, nanomechanical devices, and both crystalline and aperiodic arrays. The goal of DNA nanotechnology is control of the structure of macroscopic matter on the finest possible scale. Applications are expected to arise in the areas of X‐ray crystallography, nanoelectronics, nanorobotics, and DNA‐based computation. DNA and its close molecular relatives appear extremely well suited for these goals. This overview covers the generation of new DNA motifs, construction methods (synthesis, hybridization, phosphorylation, ligation), and a variety of methods for characterization of motifs, devices, and arrays. Finally, the use of DNA nanotechnology as a tool in biochemistry is discussed.

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Table of Contents

  • The Generation of New DNA Motifs
  • Construction Methods
  • Characterization in DNA Nanotechnology
  • Characterization of Devices
  • DNA Nanaotechnology as a Tool in Biochemistry
  • Conclusions
  • Literature Cited
  • Figures
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Literature Cited

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