Receptor Binding Techniques

Kirk A. Frey1, Roger L. Albin2

1 University of Michigan, Ann Arbor, Michigan, 2 University of Michigan and Ann Arbor Veterans' Administration Medical Center, Ann Arbor, Michigan
Publication Name:  Current Protocols in Neuroscience
Unit Number:  Unit 1.4
DOI:  10.1002/0471142301.ns0104s00
Online Posting Date:  May, 2001
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This overview first discusses issues relating to the selection of radioligand for receptor binding assays, including the isotopic label and considerations pertaining to the pharmacological and chemical profile of the ligand. This is followed by a section on characterization of ligandā€binding assays, starting with tissue preparation methods, followed by detection of specific binding, determination of incubation and washing conditions and a discussion of saturation and competition assay formats. Quantification of the assay results can be accomplished by autoradiography or film densitometry. Finally, methods and considerations for analysis of the resulting data are presented.

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Table of Contents

  • Radioligand Selection
  • Characterization of Ligand‐Binding Assays
  • Quantification of Ligand Binding
  • Analysis of Autoradiographic Data
  • Conclusion
  • Literature Cited
  • Tables
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Literature Cited

Literature Cited
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