How to Prepare Neuroanatomical Image Data

Nathan O'Connor1, Susan Tappan2, Jacob Glaser2

1 MBF Bioscience, Williston, Vermont, 2 MBF Laboratories, Williston, Vermont
Publication Name:  Current Protocols in Neuroscience
Unit Number:  Unit 1.21
DOI:  10.1002/0471142301.ns0121s69
Online Posting Date:  October, 2014
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As image data from a single neuroanatomical study can easily exceed tens of gigabytes, managing, analyzing, and presenting it is not trivial. Careful planning along multiple axes is required and includes the following: (1) Organizational methods developed for images should allow for easy and efficient access, selection, and potential reorganization of images. (2) Experimental information and other metadata should be readily available and accompany image data. (3) Even if a study's entire body of image data is made available, highlighting key results and preparing figures requires selecting image regions and resolutions, creating annotations, and adhering to publishing and community guidelines for image adjustments. Further, it may be necessary to assess Internet accessibility and infrastructure issues and to consider image formats appropriate for Web publishing. Finally, a strategy for robust, long‐term, and efficient storage of image data should be developed. This unit provides a guide for preparing neuroanatomical image data. Curr. Protoc. Neurosci. 69:1.21.1‐1.21.14. © 2014 by John Wiley & Sons, Inc.

Keywords: whole‐slide imaging; image management; virtual slides; big data; virtual tissue

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Table of Contents

  • Introduction
  • Basic Protocol 1: Naming and Organization of Images and Metadata
  • Basic Protocol 2: Preparing and Annotating Images for Presentations and Figures
  • Basic Protocol 3: Assessing Internet Environment and Optimizing Images
  • Commentary
  • Literature Cited
  • Figures
PDF or HTML at Wiley Online Library


PDF or HTML at Wiley Online Library



Literature Cited

Literature Cited
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