Basic Confocal Microscopy

Carolyn L. Smith1

1 National Institute of Neurological Disorders and Stroke, Bethesda, Maryland
Publication Name:  Current Protocols in Neuroscience
Unit Number:  Unit 2.2
DOI:  10.1002/0471142301.ns0202s56
Online Posting Date:  July, 2011
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Abstract

This unit introduces the reader to the basic principles of confocal microscopy and the design and capabilities of current confocal microscopes. The advantages and disadvantages of confocal microscopy compared to other techniques for fluorescence imaging are described. There are also practical guidelines for sample preparation and optimization of imaging parameters, as well as examples of some of the applications of confocal microscopy. Curr. Protoc. Neurosci. 56:2.2.1‐2.2.18. © 2011 by John Wiley & Sons, Inc.

Keywords: confocal microscopy; fluorescence; imaging; resolution; three‐dimensional reconstruction

     
 
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Table of Contents

  • Introduction
  • Basis of Optical Sectioning
  • Configuration of an LSCM
  • Practical Guidelines
  • Commentary
  • Literature Cited
  • Figures
  • Tables
     
 
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Materials

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Figures

Videos

Literature Cited

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Key References
   Inoué and Spring, 1997. See above.
  Covers the basics of light microscopy, video microscopy, and much more.
   Matsumoto, 2002. See above.
  Good source of practical information about confocal imaging.
   Pawley, 1995, 2006. See above.
  Two editions of comprehensive reference book on confocal microscopy.
   Russ, 2002. See above.
  Guide to digital image processing.
Internet Resources
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