Electronic Imaging in Neuroscience

Kenneth R. Spring1

1 National Institutes of Health National Heart, Lung and Blood Institute, Bethesda, Maryland
Publication Name:  Current Protocols in Neuroscience
Unit Number:  Unit 2.4
DOI:  10.1002/0471142301.ns0204s18
Online Posting Date:  May, 2002
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Abstract

This unit is intended to aid the neuroscientist in understanding the basics of image detectors and selecting a suitable camera for various neuroscience research applications. A procedure is described for evaluating cameras in the laboratory.

     
 
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Table of Contents

  • Camera Basics
  • Low‐Light‐Level Imaging of Fluorescence
  • Choosing the Appropriate Camera
  • Evaluating Camera Performance
  • Figures
     
 
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Materials

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Figures

Videos

Literature Cited

Literature Cited
   Inoué, S. and Spring, , K.R. 1997. Video Microscopy: The Fundamentals. Plenum, New York.
Key References
   Inoué and Spring 1997. See above.
  A good general reference for microscope optics and electronic imaging.
   Shotton, D. 1993. Electronic Light Microscopy. Wiley‐Liss, New York.
  This text emphasizes image acquisition, sampling and image processing.
   Sluder, G. and Wolf, D.E. 1998. Methods in Cell Biology, Volume 56, Video Microscopy. Academic Press, San Diego.
  Offers practical guidance in the acquisition of high quality electronic images of microscopic specimens.
Internet Resources
   http://microscopy.fsu.edu
  A massive collection of static and dynamic information about optics, microscopy, image processing and related topics.
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