Gene Expression in Transgenic Mice Using Neural Promoters

Shigeo Okabe1

1 Tokyo Medical and Dental University, Tokyo, Japan
Publication Name:  Current Protocols in Neuroscience
Unit Number:  Unit 3.16
DOI:  10.1002/0471142301.ns0316s07
Online Posting Date:  May, 2001
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In the first part of this unit, major considerations for the analysis of neural promoters in transgenic mice are discussed. Detailed protocols on the production of transgenic mice are not described in this unit. Advantages and disadvantages of the transgenic approach for analysis of neural cis‐acting elements are also presented. The concept of transient transgenic mice is then introduced; this method compensates for some disadvantages associated with the conventional transgenic approach. Finally, major factors influencing the efficiency of transgenic mouse production are discussed. The second part of the unit presents detailed information on a variety of neural‐specific cis‐acting elements that have been characterized by a transgenic approach. This information is useful both as a guide for carrying out the analysis of cis‐acting elements and as a reference for selection of promoter/enhancer elements for designing an appropriate transgenic construct.

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Table of Contents

  • Analysis of Neural Promoters Using Transgenic Mice
  • Neural Promoters Characterized Using the Transgenic Approach
  • Conclusion
  • Tables
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Key Reference
   Hogan et al., 1994. See above.
  The best reference for the production of transgenic mice.
   Vidal et al., 1990; Zimmerman et al., 1994
  Two good examples for the promoter/enhancer analyses using different strategies.
Internet Resources
  Web site for the database of transgenic/knockout mice. This site also has links to a variety of related Web sites.
  Web site for mouse genome database at Jackson Laboratory.
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