Overview of Membrane Protein Solubilization

Michael I. Schimerlik1

1 Oregon state University, Corvallis, Oregon
Publication Name:  Current Protocols in Neuroscience
Unit Number:  Unit 5.9
DOI:  10.1002/0471142301.ns0509s02
Online Posting Date:  May, 2001
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Abstract

The solubilization of membrane proteins (i.e., extraction of the protein from the membrane in a disperse state in aqueous solution) in a stable state that retains the native activity of the membrane‐bound form is more an empirical art than a protocol‐driven science. Although a large number of molecules have been invented that display detergent properties, selection of the proper solubilizing agent is largely a matter of trial and error that depends on the protein of interest and the particular goals of the investigator. The purpose of this overview is to describe the methodology for finding the optimal conditions for detergent solubilization and to relate the selection of a specific detergent to explicit experimental goals.

     
 
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Table of Contents

  • Selection of Detergent
  • Evaluation of Solubilization Conditions
  • Solubilization of m2 Muscarinic Receptors
  • Conclusions
  • Literature Cited
  • Tables
     
 
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Materials

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Literature Cited

Literature Cited
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