The Use of Phage Display in Neurobiology

Andrew R.M. Bradbury1

1 Los Alamos National Laboratory, Los Alamos, New Mexico
Publication Name:  Current Protocols in Neuroscience
Unit Number:  Unit 5.12
DOI:  10.1002/0471142301.ns0512s51
Online Posting Date:  April, 2010
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Phage display has been extensively used to study protein‐protein interactions, receptor‐ and antibody‐binding sites, and immune responses, to modify protein properties, and to select antibodies against a wide range of different antigens. In the format most often used, a polypeptide is displayed on the surface of a filamentous phage by genetic fusion to one of the coat proteins, creating a chimeric coat protein, and coupling phenotype (the protein) to genotype (the gene within). As the gene encoding the chimeric coat protein is packaged within the phage, selection of the phage on the basis of the binding properties of the polypeptide displayed on the surface simultaneously results in the isolation of the gene encoding the polypeptide. This unit describes the background to the technique, and illustrates how it has been applied to a number of different problems, each of which has its neurobiological counterparts. Although this overview concentrates on the use of filamentous phage, which is the most popular platform, other systems are also described. Curr. Protoc. Neurosci. 51:5.12.1‐5.12.27. © 2010 by John Wiley & Sons, Inc.

Keywords: phage; phage display; antibody; peptide; protein‐protein interactions; cDNA; genomic library

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Table of Contents

  • Filamentous Phage Biology
  • Phage Display
  • Vector Systems Used for Phage Display
  • Use of Phage‐Display Libraries to Map Protein‐Binding Sites
  • Displaying cDNA and Genomic Libraries
  • Selecting Antibodies by Phage Display
  • Improving Affinity
  • Using Phage Antibodies as a Discovery Tool
  • Disease‐Specific Phage Antibody Libraries
  • Displaying Other Proteins
  • Specific Phage Display Applications in Neurobiology
  • Literature Cited
  • Figures
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Literature Cited

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