Quantifying Recording Quality in In Vivo Striatal Recordings

Danielle M. Friend1, Caleb Kemere2, Alexxai V. Kravitz3

1 National Institute of Diabetes and Digestive and Kidney Diseases, NIH, Bethesda, Maryland, 2 Department of Electrical and Computer Engineering, Rice University, Houston, Texas, 3 National Institute of Drug Abuse, NIH, Baltimore, Maryland
Publication Name:  Current Protocols in Neuroscience
Unit Number:  Unit 6.28
DOI:  10.1002/0471142301.ns0628s70
Online Posting Date:  January, 2015
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The striatum mediates a variety of functions including movement, decision‐making, motivation, and reward learning. In vivo recording is a powerful technique that allows for the interrogation of these striatal functions while an animal is awake and behaving. Here, we describe equipment needed and general setup for performing in vivo electrophysiology experiments, data processing, and quantification of recording quality. While this protocol is focused on striatal recordings, concepts should translate to other structures as well. © 2015 by John Wiley & Sons, Inc.

Keywords: in vivo electrophysiology; striatum; recording quality; spike sorting

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Table of Contents

  • Commentary
  • Literature Cited
  • Figures
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Basic Protocol 1:

  • Beaker of saline
  • Recording device (single electrode, micro‐electrode array, silicone probe)
  • Recording chamber/Faraday cage
  • Data acquisition/analysis system
  • High‐pass and low‐pass filters
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Literature Cited

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