Overview of Methodological Approaches to the Study of Ingestive Behavior

Bartley G. Hoebel1, Paul J. Wellman2

1 Princeton University, Princeton, New Jersey, 2 Texas A&M University, College Station, Texas
Publication Name:  Current Protocols in Neuroscience
Unit Number:  Unit 8.6A
DOI:  10.1002/0471142301.ns0806as03
Online Posting Date:  May, 2001
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Abstract

Some examples of procedures used by feeding researchers are discussed in this unit and include ablation of neural function; inhibition of behaviors by selective neurotoxins and antisense oligonucleotides; staining of sensory and motor mechanisms; electrical stimulation of the brain; local injection and microdialysis of nutrients, neurotransmitters, and drugs; autoradiography and in situ hybridization of neurotransmitters with their receptors; electrophysiological techniques for multi‐ and single‐unit recording of cells in the hypothalamus; and gene technology using inbred strains of genetically obese mice.

     
 
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Table of Contents

  • Tissue Inactivation
  • Treatment with Selective Neurotoxins and Antisense Oligonucleotides
  • Staining
  • Electrical Stimulation
  • Local Injection and Microdialysis
  • Autoradiography and In Situ Hybridization
  • Electrophysiological Techniques
  • Gene Technology
  • Further Directions in Ingestive Behavior Research
  • Literature Cited
     
 
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Materials

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Literature Cited

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Key References
   Bray, G.A. and Ryan, D.H. (eds.) 1996. Molecular and Genetic Aspects of Obesity. The Pennington Center Nutrition Series, Vol.5. LSU Press, Baton Rouge, La.
  This volume of 37 chapters considers human disease states and animal models of obesity with specific focus on genetic and molecular aspects, including neural mechanisms underlying eating and body weight regulation.
   Cooper, S.J. and Clifton, P.G. (eds.) 1996. Drug Receptor Subtypes and Ingestive Behavior. Academic Press, London.
  This volume of 17 chapters deals with central and peripheral receptors involved in eating and energy balance, including receptors for CCK, serotonin, opioids, dopamine, NPY, glutamate, and benzodiazepines.
   Hoebel, 1997. See above.
  A review of procedures and progress in the neuroscience of ingestive behavior.
   Legg, C.R. and Booth, D.D. (eds.) 1994. Appetite: Neural and Behavioral Bases. Oxford University Press, Oxford.
  A thorough treatment of neural and learning factors in feeding.
   Toates, F.M. and Rowland, N.E. (eds.) 1987. Feeding and drinking. In Techniques in the Behavioral and Neural Sciences, Vol. 1. Elsevier, New York.
  A comprehensive treatment of issues, methods, and procedures for studies of ingestion in animals and humans.
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