Overview of Methods for Assessing the Mouse Auditory System

James F. Willott1

1 University of South Florida and The Jackson Laboratory, Tampa, Florida
Publication Name:  Current Protocols in Neuroscience
Unit Number:  Unit 8.21A
DOI:  10.1002/0471142301.ns0821as34
Online Posting Date:  February, 2006
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Abstract

The three general approaches for assessing the mouse auditory system presented in UNITS– are complementary, but differ in the time required for testing as well as the goals of the tests: the auditory brainstem response (ABR; UNIT), distortion product otoacoustic emissions (DPOAE; UNIT), and lick suppression behavioral test (UNIT). Various issues that need to be addressed in the testing of mouse hearing are discussed in this overview, including accurate nomenclature of the strain and/or mutations, environmental versus genetic influences, selection of frequencies to be tested, and appropriate animal care and use.

Keywords: ABR; DPOAE; psychophysics; inbred strains; audiogram; mouse

     
 
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Table of Contents

  • Issues to Consider when Studying Mice
  • What Frequencies Should be Tested?
  • Approval of Protocol
  • Literature Cited
     
 
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Materials

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Figures

Videos

Literature Cited

   Bohne, B.A., Harding, G.W., and Ou, H.C. 2001. Preparation and evaluation of the mouse temporal bone. In Handbook of Mouse Auditory Research: From Behavior to Molecular Biology (J.F. Willott, ed.) pp. 171‐188. CRC Press, Boca Raton, Fla.
   Ehret, G. 1983. Peripheral anatomy and physiology II. In Auditory Psychobiology of the Mouse (J.F. Willott, ed.) pp. 169‐200. Charles C. Thomas, Springfield, Ill.
   Fay, R.R. 1988. Hearing in Vertebrates: A Psychophysics Databook, Hill‐Fay, Winnetka, Ill.
   Nadol, J.B., Jr. 1981. The aging peripheral hearing mechanism. In Aging: Communication Processes and Disorders (D.S. Beasley and G.A. Davis, eds.) pp. 63‐85. Grune & Stratton, New York.
Key References
   Parham, K., Sun, X.‐M., and Kim, D.O. 2001. Noninvasive assessment of auditory function: Distortion product otoacoustic emissions and auditory brainstem responses. In Handbook of Mouse Auditory Research: From Behavior to Molecular Biology (J.F. Willott, ed.) pp. 37‐58. CRC Press, Boca Raton, Fla.
  More on electrophysiological methods.
   Heffner, H. and Heffner, R.S. 2001. Behavioral assessment of hearing in mice. In Handbook of Mouse Auditory Research: From Behavior to Molecular Biology (J.F. Willott, ed.) pp. 19‐30. CRC Press, Boca Raton, Fla.
  More on behavioral tests.
   Willott, J.F. (ed.). 1983. The Auditory Psychobiology of the Mouse, Charles C. Thomas Publishers, Springfield, Ill.
  Summary of early research.
   Willott, J.F. (ed.). 2001. Handbook of Mouse Auditory Research: From Behavior to Molecular Biology, CRC Press, Boca Raton, Fla.
  Summary of recent research.
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