Systems Genetic Analysis in

Clarissa C. Parker1, Price E. Dickson2, Vivek M. Philip3, Mary Thomas1, Elissa J. Chesler2

1 Department of Psychology and Program in Neuroscience, Middlebury College, Middlebury, Vermont, 2 Center for Mammalian Genetics, The Jackson Laboratory, Bar Harbor, Maine, 3 Center for Computational Sciences, The Jackson Laboratory, Bar Harbor, Maine
Publication Name:  Current Protocols in Neuroscience
Unit Number:  Unit 8.39
DOI:  10.1002/cpns.23
Online Posting Date:  April, 2017
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Genome‐wide association studies (GWAS) have emerged as a powerful tool to identify alleles and molecular pathways that influence susceptibility to psychiatric disorders and other diseases. Forward genetics using mouse mapping populations allows for a complementary approach that provides rigorous genetic and environmental control. In this unit, we describe techniques and tools that reduce the technical burden traditionally associated with genetic mapping in mice and enhance their translational utility to human psychiatric disorders. We provide guidance on choosing the appropriate mapping population, discuss the importance of phenotype, and offer detailed instructions on using the Web‐based resource GeneNetwork to aid neuroscientists in better understanding the mechanisms through which genes influence behavior. We believe that the continued development of mouse mapping populations, genetic tools, bioinformatics resources, and statistical methodologies should remain a parallel strategy by which to investigate the genetic and environmental underpinnings of psychiatric disorders and other diseases in humans. © 2017 by John Wiley & Sons, Inc.

Keywords: GeneNetwork; quantitative trait locus mapping; recombinant inbred strains; systems genetics

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Table of Contents

  • Introduction
  • Strategic Planning
  • Basic Protocol 1: Genetic Mapping and Systems Genetics Using GeneNetwork
  • Support Protocol 1: Composite Interval Mapping Using GeneNetwork
  • Commentary
  • Literature Cited
  • Figures
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Key Reference
  Williams, R.W. and Williams, E.G. 2016. See above.
  This is an excellent review that is intended to guide researchers through the process of designing mapping experiments and choosing appropriate mapping populations given the type of question under investigation.
Internet Resource
  GeneNetwork is a free scientific Web resource used to study relationships between differences in genes, environmental factors, phenotypes, and disease risk.
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