Optimization of Transfection

John K. Rose1

1 Yale University School of Medicine, New Haven, Connecticut
Publication Name:  Current Protocols in Neuroscience
Unit Number:  Appendix 1B
DOI:  10.1002/0471142301.nsa01bs01
Online Posting Date:  May, 2001
GO TO THE FULL TEXT: PDF or HTML at Wiley Online Library

Abstract

The single most important factor in optimizing transfection efficiency is selecting the proper transfection protocol. This overview discusses the most commonly used procedures: calcium phosphate‐mediated gene transfer, DEAE‐dextran‐mediated gene transfer, electroporation, and liposome‐mediated transfection. Fusion techniques such as protoplast fusion and microinjection may also be considered.

     
 
GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online Library

Table of Contents

  • Calcium Phosphate Transfection
  • DEAE‐Dextran Transfection
  • Electroporation
  • Liposome‐Mediated Transfection
  • Tables
     
 
GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online Library

Materials

GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online Library

Figures

Videos

Literature Cited

GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online Library