Common Stock Solutions, Buffers, and Media


Publication Name:  Current Protocols in Neuroscience
Unit Number:  Appendix 2A
DOI:  10.1002/0471142301.nsa02as00
Online Posting Date:  May, 2001
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Abstract

    Recipes

  • Acid, concentrated stock solutions

  • Ammonium hydroxide, concentrated stock solution

  • Artificial cerebrospinal fluid (aCSF)

  • Calcium‐ and magnesium‐free Hank's buffered salt solution (CMF‐HBSS)

  • Denhardt's solution, 100×

  • Diethylpyrocarbonate (DEPC) treatment of solutions

  • DNase I, RNase‐free (1 mg/ml)

  • Dulbecco's modified Eagle medium (DMEM), supplemented

  • EDTA (ethylenediaminetetraacetic acid), 0.5 M (pH 8.0)

  • Ethidium bromide staining solution

  • Fetal bovine serum (FBS)

  • Gel loading buffer, 6×

  • HBSS (Hanks' balanced salt solution)

  • LB medium (Luria broth) and LB plates

  • PBS (phosphate‐buffered saline)

  • Phenol, buffered

  • Phenol/chloroform/isoamyl alcohol, 25:24:1 (v/v/v)

  • Polylysine‐coated tissue culture surfaces

  • Potassium phosphate buffer, 0.1 M

  • RNase a stock solution, DNase‐free, 2 mg/ml

  • SDS sample buffer (for discontinuous systems)

  • Sodium acetate, 3 M

  • Sodium phosphate buffer, 0.1 M

  • SSC (sodium chloride/sodium citrate), 20×

  • SSPE (sodium chloride/sodium phosphate/EDTA), 20×

  • TBE (Tris/borate/EDTA) electrophoresis buffer, 10×

  • TE (Tris/EDTA) buffer

  • Tris‐buffered saline (TBS)

  • Tris⋅Cl, 1 M

     
 
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Table of Contents

  • Literature Cited
  • Tables
     
 
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Materials

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Figures

Videos

Literature Cited

Literature Cited
   Chemical Rubber Company. 1975. CRC Handbook of Biochemistry and Molecular Biology, Physical and Chemical Data, 3d ed., Vol. 1. CRC Press, Boca Raton, Fla.
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