Parenteral Injections

John Donovan1, Patricia Brown2

1 Wyeth‐Ayerst Research, Collegeville, Pennsylvania, 2 National Institutes of Health, Bethesda, Maryland
Publication Name:  Current Protocols in Neuroscience
Unit Number:  Appendix 4F
DOI:  10.1002/0471142301.nsa04fs33
Online Posting Date:  November, 2005
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Abstract

This unit describes the techniques for the following routes of injection for mice, rats, hamsters and rabbits: intramuscular, intradermal, subcutaneous, intravenous, intraperitoneal, footpad, and intrathymic. Guidelines are also given regarding injection volumes and temperatures, and the use of proper restraints.

     
 
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Table of Contents

  • Basic Protocol 1: Intramuscular Injection of Mouse, Rat, Hamster, and Rabbit
  • Basic Protocol 2: Intradermal Injection of Mouse, Rat, Hamster, and Rabbit
  • Basic Protocol 3: Subcutaneous Injection of Mouse, Rat, Hamster, and Rabbit
  • Basic Protocol 4: Intravenous Injection of the Mouse
  • Basic Protocol 5: Intravenous Injection of the Rat
  • Basic Protocol 6: Intravenous Injection of the Hamster
  • Basic Protocol 7: Intravenous Injection of the Rabbit
  • Basic Protocol 8: Intraperitoneal Injection of Mouse, Rat, Hamster, and Rabbit
  • Basic Protocol 9: Footpad Injection of the Mouse
  • Basic Protocol 10: Intrathymic Injection of the Mouse
  • Figures
  • Tables
     
 
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Materials

Basic Protocol 1: Intramuscular Injection of Mouse, Rat, Hamster, and Rabbit

  Materials
  • Injectate
  • 1‐ to 3‐ml syringe with 22‐ to 30‐G needle

Basic Protocol 2: Intradermal Injection of Mouse, Rat, Hamster, and Rabbit

  Materials
  • 70% ethanol
  • Injectate
  • Clippers with #40 blade
  • Gauze sponge or swab
  • 1‐ to 3‐ml syringe with 25‐ to 30‐G needle
  • Additional reagents and equipment for handling and restraint ( appendix 4D) and anesthesia ( appendix 4B)

Basic Protocol 3: Subcutaneous Injection of Mouse, Rat, Hamster, and Rabbit

  Materials
  • Injectate
  • 1‐ to 3‐ml syringe with 22‐ to 30‐G needle

Basic Protocol 4: Intravenous Injection of the Mouse

  Materials
  • Injectate
  • 70% ethanol
  • 1‐ml syringe with 25‐ to 30‐G needle
  • Restrainer ( appendix 4D)
  • Heat lamp or beaker containing warm water
  • Gauze sponge or swab

Basic Protocol 5: Intravenous Injection of the Rat

  Materials
  • Injectate
  • 1‐ml syringe with 25‐ to 30‐G needle
  • Additional reagents and equipment for anesthesia ( appendix 4B)

Basic Protocol 6: Intravenous Injection of the Hamster

  Materials
  • 70% ethanol
  • Injectate
  • Clippers with #40 blade
  • Gauze sponge or swab
  • 1‐ml syringe with 25‐ to 30‐G needle
  • Additional reagents and equipment for anesthesia ( appendix 4B)

Basic Protocol 7: Intravenous Injection of the Rabbit

  Materials
  • 70% ethanol
  • Injectate
  • Restrainer ( appendix 4D)
  • Gauze sponge or swab
  • 1‐ or 3‐ml syringe with 25‐ to 30‐G butterfly infusion set

Basic Protocol 8: Intraperitoneal Injection of Mouse, Rat, Hamster, and Rabbit

  Materials
  • Injectate
  • 1‐ or 3‐ml syringe with 22‐ to 25‐G needle (mouse, rat, and hamster) or 18‐ to 23‐G, 1‐in. needle (rabbit)

Basic Protocol 9: Footpad Injection of the Mouse

  Materials
  • Injectate
  • 1‐ml syringe with 25‐G needle

Basic Protocol 10: Intrathymic Injection of the Mouse

  Materials
  • 70% ethanol
  • Injectate
  • Surgical board
  • Gauze sponge or swab
  • Scalpel handle (#3) and scalpel blade (#15)
  • Thumb forceps
  • Iris scissors, straight
  • 1‐ml syringe with 28‐ to 30‐G needle
  • Wound clips or 4‐0 suture material with needle
  • Additional reagents and equipment for anesthesia ( appendix 4B)
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Figures

Videos

Literature Cited

Literature Cited
   Tuffery, A.A. 1987. Laboratory Animals: An Introduction for New Experimenters, pp. 225‐226. John Wiley & Sons, Chichester, England.
   Waynforth, H.B. 1980. Experimental and Surgical Technique in the Rat, p. 39. Academic Press, Orlando.
Key References
   Fox, J.G., Anderson, L.C., Loew, F.M., and Quimby, F.W. (eds.). 2002. Laboratory Animal Medicine, 2nd ed. Academic Press, Orlando, Fla.
  Provides additional details for injection methods.
   Goldschneider, I., Komschlies, K.L., and Greiner, D.L. 1986. Studies of thymocytes in rats and mice. J. Exp. Med. 163:1‐17.
  Provides detailed description of the intrathymic injection technique.
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