Blood Collection

John Donovan1, Patricia Brown2

1 Wyeth‐Ayerst Research, Collegeville, Pennsylvania, 2 National Institutes of Health, Bethesda, Maryland
Publication Name:  Current Protocols in Neuroscience
Unit Number:  Appendix 4G
DOI:  10.1002/0471142301.nsa04gs33
Online Posting Date:  November, 2005
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Abstract

Blood is most frequently sampled for evaluation of serum antibodies or analysis of surface markers on peripheral blood cells. The protocols offered in this unit describe collection of blood from the orbital sinus or plexus of the mouse, rat, or hamster. With appropriate techniques, small amounts of blood can be obtained with little ill effect on the animal. Collection from the auricular vein or artery of the rabbit is also relatively unstressful to the animal. Bleeding procedures that should be performed on the anesthetized animal include collection from the mouse axillary plexus, cardiac puncture of the mouse, rat, hamster, or rabbit, and collection from the hamster abdominal aorta or vena cava.

     
 
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Table of Contents

  • Basic Protocol 1: Blood Collection from Orbital Sinus or Plexus of Mouse and Rat
  • Basic Protocol 2: Blood Collection from Tail Vein of Mouse and Rat Using Microhematocrit Tube
  • Alternate Protocol 1: Blood Collection from Tail Vein of Mouse and Rat Using Centrifuge Tube
  • Basic Protocol 3: Blood Collection from Axillary Plexus of Mouse
  • Basic Protocol 4: Cardiac Puncture of Mouse and Rat
  • Basic Protocol 5: Blood Collection from Hamster Orbital Sinus
  • Basic Protocol 6: Cardiac Puncture of Hamster
  • Basic Protocol 7: Blood Collection from Hamster Abdominal Aorta/Vena Cava
  • Basic Protocol 8: Blood Collection from Marginal Ear Vein of Rabbit
  • Basic Protocol 9: Blood Collection from Auricular Artery of Rabbit
  • Basic Protocol 10: Cardiac Puncture of Rabbit
  • Basic Protocol 11: Blood Collection from the Saphenous Vein of Mouse, Rat, and Hamster
  • Basic Protocol 12: Blood Collection from the Facial Vein of the Mouse
  • Commentary
  • Literature Cited
  • Figures
  • Tables
     
 
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Materials

Basic Protocol 1: Blood Collection from Orbital Sinus or Plexus of Mouse and Rat

  Materials
  • Sterile saline or phosphate‐buffered saline (PBS; appendix 2A)
  • Microhematocrit tube
  • Gauze sponge or swab
  • Additional reagents and equipment for handling and restraint ( appendix 4D) and anesthesia ( appendix 4B)

Basic Protocol 2: Blood Collection from Tail Vein of Mouse and Rat Using Microhematocrit Tube

  Materials
  • Heat lamp or beaker containing warm water
  • 25‐ to 30‐G needle
  • Microhematocrit tube
  • Gauze sponge
  • Additional reagents and equipment for handling and restraint ( appendix 4D)

Alternate Protocol 1: Blood Collection from Tail Vein of Mouse and Rat Using Centrifuge Tube

  • Scalpel blade (#10)
  • 12‐ to 50‐ml plastic centrifuge tube

Basic Protocol 3: Blood Collection from Axillary Plexus of Mouse

  Materials
  • Surgical board
  • Scalpel or razor blade
  • Thumb forceps
  • Surgical scissors
  • Additional reagents and equipment for anesthesia ( appendix 4D)

Basic Protocol 4: Cardiac Puncture of Mouse and Rat

  Materials
  • 1‐ to 3‐ml syringe (mouse) or 3‐ to 10‐ml syringe (rat), with 20‐ to 22‐G needle
  • Additional reagents and equipment for anesthesia ( appendix 4B)

Basic Protocol 5: Blood Collection from Hamster Orbital Sinus

  Materials
  • Microhematocrit tube or Pasteur pipet
  • 12‐ to 15‐ml centrifuge tube
  • Additional reagents and equipment for anesthesia ( appendix 4B)

Basic Protocol 6: Cardiac Puncture of Hamster

  Materials
  • 1‐ml syringe with 23‐G needle
  • Additional reagents and equipment for anesthesia ( appendix 4B)

Basic Protocol 7: Blood Collection from Hamster Abdominal Aorta/Vena Cava

  Materials
  • #10 scalpel blade with handle
  • 5‐ml syringe with 23‐ to 25‐G needle
  • Additional reagents and equipment for anesthesia ( appendix 4B)

Basic Protocol 8: Blood Collection from Marginal Ear Vein of Rabbit

  Materials
  • 70% ethanol
  • 40% solution d‐limonene in 95% ethanol
  • Gauze sponge or swab
  • Scalpel blade
  • 12‐ to 50‐ml centrifuge tube
  • Additional reagents and equipment for handling and restraint ( appendix 4D) or sedation

Basic Protocol 9: Blood Collection from Auricular Artery of Rabbit

  Materials
  • 70% ethanol
  • Gauze sponge or swab
  • 5‐ to 60‐ml syringe with 21‐ to 22‐G butterfly infusion set
  • Additional reagents and equipment for handling and restraint ( appendix 4D) or sedation

Basic Protocol 10: Cardiac Puncture of Rabbit

  Materials
  • Ketamine/xylazine solution ( appendix 4B)
  • 70% ethanol
  • Gauze sponge or swab
  • 5‐ to 60‐ml syringe with 18‐ to 25‐G, 1.5‐in. needle

Basic Protocol 11: Blood Collection from the Saphenous Vein of Mouse, Rat, and Hamster

  Materials
  • 50‐ml polypropylene conical centrifuge tubes
  • Towel
  • 20‐ to 22‐G needle
  • Microhematocrit tube
  • Gauze sponge

Basic Protocol 12: Blood Collection from the Facial Vein of the Mouse

  Materials
  • GoldenRod animal lancet (MEDIpoint; http://www.medipoint.com) or 18‐ to 22‐G needle
  • Capillary blood collection tube
  • Gauze sponge
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Figures

Videos

Literature Cited

   Gay, W.I. 1986. Methods of Animal Experimentation, Vol. VII, pp. 31‐65. Academic Press, San Diego.
   Tuffery, A.A. 1987. Lab Animals: An Introduction for New Experimenters, p. 248. John Wiley & Sons, Chichester, England.
Key References
   Hem, A., Smith, A.J., and Soberg, P. 1998. Saphenous vein puncture for blood sampling of the mouse, rat, hamster, gerbil, guinea pig, ferret, and mink. Lab. Anim. 32:364‐368.
  Expanded description of blood collection from saphenous vein.
   http://www.lal.org.uk/pdffiles/LAB1607.PDF.
  Additional details for rabbit blood and collection methods.
   Manning, P.J., Ringler, D.H. and Newcomer, C.E. (eds.) 1994. The Biology of the Laboratory Rabbit, 2nd ed., ACLAM Series. pp. 73‐74. Academic Press, San Diego.
  Detailed description of hamster orbital sinus blood collection.
   Van Hoosier, G.L. and MacPherson, C.W. 1987. Laboratory Hamsters, pp. 75‐76. Academic Press, Orlando.
Internet Resources
  http://www.uib.no/vivariet/mou_blood/Blood_coll_mice_.html
  Provides detailed description and illustrations of saphenous blood collection.
  http://www.medipoint.com/html/animal_lancets.html
  Depicts animal lancet use and anatomical landmarks for facial vein blood collection.
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