Isolated Blood Vessel Assays

Terry Kenakin1

1 Glaxo Wellcome Research and Development, Research Triangle Park, North Carolina
Publication Name:  Current Protocols in Pharmacology
Unit Number:  Unit 4.4
DOI:  10.1002/0471141755.ph0404s00
Online Posting Date:  May, 2001
GO TO THE FULL TEXT: PDF or HTML at Wiley Online Library

Abstract

One of the most versatile and accessible types of isolated tissue used in pharmacology is the isolated blood vessel. Arteries and veins contain a wide range of receptor types mediating contraction, relaxation, and endothelial function. The protocols in this unit describe methods for measuring agonist response as changes in blood vessel tension.

     
 
GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online Library

Table of Contents

  • Basic Protocol 1: Ring Preparations of Blood Vessels
  • Alternate Protocol 1: Spiral Strip Preparations of Blood Vessels
  • Basic Protocol 2: Perfused Preparations of Blood Vessels
  • Commentary
  • Figures
     
 
GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online Library

Materials

Basic Protocol 1: Ring Preparations of Blood Vessels

  Materials
  • Isolated blood vessel (see )
  • Physiological salt solution (PSS; unit 4.3) or equivalent, 4° and 37°C, oxygenated by bubbling with carbogen gas (95% O 2/5% CO 2)
  • 0.1 M KCl stock solution
  • Polyethylene cannula or rolled tubing, size appropriate for vessel internal diameter (Fisher)
  • Hooks with ends bent at right angles (see Fig. )
  • Organ bath heated to 37°C and bubbled with carbogen gas
  • Isometric transducer (Grass FT.03 or equivalent)

Alternate Protocol 1: Spiral Strip Preparations of Blood Vessels

  • Rat
  • Sterile dissection instruments:
  •  Scalpel
  •  Forceps
  •  Fine scissors
  • Stainless steel syringe needle or polyethylene cannula (gauge appropriate for internal diameter of the vessel)
  • Plexiglas tissue holder (Harvard Apparatus; see unit 4.3)
  • 5‐0 silk thread

Basic Protocol 2: Perfused Preparations of Blood Vessels

  Materials
  • Male rabbit, 2.5 to 4.0 kg
  • Physiological salt solution (PSS; see unit 4.3) or equivalent, 4° and 37°C, oxygenated by bubbling with carbogen gas (95% O 2/5% CO 2).
  • 25% urethane solution
  • 0.1 M KCl stock solution
  • Perfusion apparatus (see Fig. C).
  • Sterile scalpel, forceps, fine scissors
  • Tissue holder (see Fig. )
  • Polyethylene cannula of appropriate diameter
  • Organ bath heated to 37°C
GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online Library

Figures

Videos

Literature Cited

Literature Cited
   Altura, B.M. and Altura, B.T. 1970. Heterogeneity of drug receptors in different segments of rabbit thoracic aorta. Eur. J. Pharmacol. 12:44‐52.
   de la Lande, I.S. and Jellett, L.B. 1972. Relationship between the roles of monoamine oxidase and sympathetic nerves in the vasoconstrictor response of the rabbit ear artery to norepinephrine. J. Pharmacol. Exp. Ther. 180:47‐55.
   de la Lande, I.S. and Rand, M.J. 1965. A simple isolated nerve‐blood vessel preparation. Aust. J. Exp. Biol. Med. Sci. 43:639‐656.
   Hooker, C.S., Calkins, P.J., and Fleisch, J.H. 1977. On the measurement of vascular and respiratory smooth muscle response in vitro. Blood Vessels 14:1‐11.
   Langendorf, O. 1895. Untersuchungen am uberlebenden Saugethierherzen. Pflug. Arch. Ges. Physiol. 61:291‐332.
Key Reference
   University of Edinburgh Staff, Department of Physiology. 1968. Pharmacological Experiments on Isolated Preparations. E. & S. Livingstone Ltd., Edinburgh.
  A comprehensive volume written by specialists in this field, detailing the preparation of numerous blood vessels.
GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online Library