Models of Inflammation: Adjuvant‐Induced Arthritis in the Rat

Phyllis E. Whiteley1, Stacie A. Dalrymple1

1 Roche Bioscience, Palo Alto, California
Publication Name:  Current Protocols in Pharmacology
Unit Number:  Unit 5.5
DOI:  10.1002/0471141755.ph0505s13
Online Posting Date:  August, 2001
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Abstract

Injection of adjuvant (Mycobacterium butyricum suspended in mineral oil) into rats produces an immune reaction that characteristically involves inflammatory destruction of cartilage and bone of the distal joints with concomitant swelling of surrounding tissues. Adjuvantā€induced arthritis in rats is commonly used to evaluate compounds that might be of potential use as drugs for treatment of rheumatoid arthritis and other chronic inflammatory conditions. This unit describes a method for inducing arthritis by injecting adjuvant into the tail and evaluating a test compound for the ability to inhibit the inflammatory response.

     
 
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Table of Contents

  • Reagents and Solutions
  • Commentary
  • Figures
  • Tables
     
 
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Materials

Basic Protocol 1:

  Materials
  • Female LEW/CrlBR (Lewis, Charles River Labs) or Crl:CD(SD)IGS BR rats weighing 90 to 110 g at the time of arrival (Sprague Dawley, Charles River Labs)
  • Adjuvant suspension (see recipe)
  • Test compound (new chemical entity, NCE)
  • CO 2 supply
  • Scales accurate to 0.1 and 0.0001 g
  • 1‐cc tuberculin syringe with Luer‐Lok tip (Becton Dickinson Labware)
  • 25‐G, ⅝‐in. needles (Becton Dickinson Labware)
  • Animal restrainer (e.g., Broom restraint, Plas Labs)
  • Guillotine (Harvard Apparatus)
  • Weigh boats
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Figures

Videos

Literature Cited

Literature Cited
   Billingham, M.E.J. 1990. Models of arthritis and the search for anti‐arthritic drugs. In Anti‐Rheumatic Drugs (M.C.L.E. Orme, ed.) pp.1‐47. Pergamon Press, Elmsford, N.Y.
   Billingham, M.E.J. 1995. Adjuvant arthritis: The first model. In Mechanisms and Models in Rheumatoid Arthritis (B. Henderson, J.C.W. Edwards, E.R. Pettipher, eds.) pp. 389‐410. Academic Press, San Diego.
Key References
   Billingham, 1990. See above.
  A review that includes models of arthritis in other species, with special attention to drug evaluation in these models.
   Billingham, 1995. See above.
  A complete review of the history of adjuvant arthritis in the rat.
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