Electrical Amygdala Kindling

N. Dürmüller1, R.D. Porsolt1

1 Porsolt and Partners Pharmacology, Boulogne Billancourts
Publication Name:  Current Protocols in Pharmacology
Unit Number:  Unit 5.33
DOI:  10.1002/0471141755.ph0533s22
Online Posting Date:  November, 2003
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Abstract

This unit describes a method of electrical amygdala kindling in the rat. This procedure requires mastery of stereotaxic electrode implantation which is not covered in the current unit. Also, the investigator must have a sound knowledge of electronics and computing. The text gives instructions on how to render rats epileptic, how to determine the effects of compounds in kindled rats, and how to analyze the data. Results with three reference substances are illustrated. These substances are used in the clinic and give robust results in kindling.

Keywords: chronically implanted rats; electrical stimulation; complex partial seizures; kindling

     
 
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Table of Contents

  • Basic Protocol 1: Electrical Amygdala Kindling in the Rat
  • Support Protocol 1: Acquisition of Amygdala Kindling Data
  • Support Protocol 2: Data Analysis for Electrical Amygdala Kindling in Rats
  • Commentary
  • Literature Cited
  • Figures
  • Tables
     
 
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Materials

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Figures

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Literature Cited

Literature Cited
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   Albright, P.S. 1983. Effects of carbamazepine, clonazepam, and phenytoin on seizure threshold in amygdala and cortex. Exp. Neurol. 79:11‐17.
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   Cain, D.P. 1983. Bidirectional transfer of electrical and carbachol kindling. Brain Res. 260:135‐138.
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