Models of Visceral Pain: Colorectal Distension (CRD)
The visceromotor response to balloon distension of the colon is a robust behavioral model of visceral nociception in rodents and is ideally suited for studying the visceral antinociceptive activity of drugs. This unit describes, in detail, quantification of this response with the use of electromyography in both rats and mice.
Keywords: Electromyography; Visceromotor; Colon; Balloon; Mouse; Rat
Table of Contents
- Basic Protocol 1: EMG Electrode Implantation in the Rat
- Alternate Protocol 1: EMG Electrode Implantation in the Mouse
- Basic Protocol 2: Preparing Rats for CRD
- Alternate Protocol 2: Preparing MICE for CRD
- Basic Protocol 3: CRD Testing Paradigms
- Literature Cited
Basic Protocol 1: EMG Electrode Implantation in the Rat
Alternate Protocol 1: EMG Electrode Implantation in the Mouse
Basic Protocol 2: Preparing Rats for CRD
Alternate Protocol 2: Preparing MICE for CRD
Basic Protocol 3: CRD Testing Paradigms
Figure 5.36.1 Rat (A) and mouse (B) EMG recording electrodes prior to implantation (top) and depicted after implantation (middle and bottom).
Figure 5.36.2 Overnight inflation of rat CRD balloons increases balloon compliance. Both balloons are inflated to ∼30 mmHg. The top balloon was inflated to ∼80 mmHg during the previous 12 hr, whereas, the bottom balloon was not.
Figure 5.36.3 CRD balloons for rat (top) and mouse (middle with sheath and bottom without sheath in place).
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