Dog EEG for Wake‐Promotion Studies

Régis Parmentier1, Denis Bricout1, Emmanuel Brousseau1, Thierry Giboulot1

1 Centre de Développement Cephalon France, Maisons Alfort
Publication Name:  Current Protocols in Pharmacology
Unit Number:  Unit 5.43
DOI:  10.1002/0471141755.ph0543s34
Online Posting Date:  October, 2006
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Described in this unit is a protocol for investigating the wake‐promoting activity of new chemical entities (NCEs) in dog. The experimental approach is based on scoring of sleep/wake stages in animals implanted with a telemetry device for recording EMG and cortical EEG signals. A major advantage of this procedure is that it is conducted in nontethered animals, limiting possible bias and complications encountered with conventional recording systems. In this procedure, polygraphic recording is conducted using four implanted beagles. Results of studies with modafinil, a wake‐promoting agent, are described to demonstrate the utility of this test procedure.

Keywords: sleep‐wake cycle; dog; EEG; wake promoting activity; animal models

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Table of Contents

  • Strategic Planning
  • Support Protocol 1: Behavioral Effects Testing
  • Reagents and Solutions
  • Commentary
  • Literature Cited
  • Figures
  • Tables
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Basic Protocol 1:

  • Amoxicillin/clavulanic acid (e.g., Synulox, Pfizer), 250‐mg tablets
  • Tolfenamic acid (e.g., Tolfedine, Vetoquinol), 60‐mg tablets
  • 12‐month‐old male and/or female beagle dogs (CEDS France), 10 to 12 kg
  • 0.15 ml/kg ketamine (e.g., Imalgene 1000; Merial)
  • 5% (v/v) lidocaine (e.g., Xylocaine; Astra Zeneca)
  • Isofluran (e.g., Forene, Abbott)
  • 0.9% NaCl/Betadine solution (see recipe)
  • Dental cement (Super‐Bond, Sun Medical)
  • Bandage seals
  • Test compound suspension in ORA‐Plus (see recipe)
  • Electric razor
  • 15‐mm intubation tube
  • Capnograph (POET TE 602‐11, Criticare)
  • Oxymeter (Dinamap Pro 400)
  • Anesthesia machine with vaporizer for isoflurane (Alpha 30, Minerve)
  • Homeothermic blanket unit (Harvard)
  • Implant: Biopotentials telemetry units (D70‐EEE, Transoma Medical; includes electrodes and ancillary material)
  • Flexidene 3/0 dec. 2 (B/Braun)
  • Mersutures 0 dec. 3.5 (Ethicon)
  • 0.8‐mm‐diameter dental burr and dental hand piece
  • Surgical screw (part no. 00‐96x3/32, Plastics one)
  • Safil 3/0 dec. 2 (B/Braun)
  • Cannula for oral dosing (Dahlhausen)
  • 2 × 2–m sound‐proofed telemetry boxes (Pajon)
  • Telemetry data acquisition system (Transoma Medical)
  • Sleep/wake analysis software (Sleepsign, Kissei Comtec)
NOTE: Conduct surgery under aseptic conditions, using autoclaved instruments, gloves, surgery trousers, and sterile fields.
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Literature Cited

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