Animal Models of Collagen‐Induced Arthritis

Stéphanie Wagner1, Julien Bindler1, Emile Andriambeloson1

1 Neurofit SAS, Illkirch, France
Publication Name:  Current Protocols in Pharmacology
Unit Number:  Unit 5.51
DOI:  10.1002/0471141755.ph0551s43
Online Posting Date:  December, 2008
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Abstract

Collagen‐induced arthritis in rats is associated with inflammatory polyarthritis, sharing clinical and pathological features with those of human rheumatoid arthritis (RA). Described in this unit is a protocol for consistently inducing arthritis in female Lewis rats by immunizing them with bovine type II collagen (CII) emulsified in complete Freund's adjuvant. This model is of value not only in defining the underlying pathogenesis of RA, but also as a tool for evaluating pharmacological strategies for treating this condition. Curr. Protoc. Pharmacol. 43:5.51.1‐5.51.8. © 2008 by John Wiley & Sons, Inc.

Keywords: rheumatoid arthritis; collagen; animal model; inflammation; rat

     
 
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Table of Contents

  • Commentary
  • Literature Cited
  • Figures
     
 
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Materials

Basic Protocol 1:

  Materials
  • 2 mg/ml type II bovine collagen (CII) stock solution in 0.05 M acetic acid (MD Biosciences)
  • Female Lewis rats (Janvier or Harlan; 8 to 11 weeks old, 140 to 180 g)
  • Complete Freund's adjuvant (CFA; Sigma cat. no. F5881)
  • Ketamine (Imalgene, Merial, http://www.merial.com) and xylazine (Rompun, Bayer Healthcare)
  • Test compounds (e.g., immunosuppressant, anti‐inflammatory, or antioxidant drugs; see for considerations regarding timepoint of administration)
  • Rat cages
  • Permanent ink marker to identify animals
  • 20‐ml glass bottle
  • 1‐ml syringes
  • 25‐G, 5/8‐in. length (0.5 × 16–mm) needles (Terumo, http://www.terumomedical.com)
  • 100‐ or 250‐ml beaker
  • Animal balance accurate to 0.01 g
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Figures

Videos

Literature Cited

Literature Cited
   Campo, G.M., Avenoso, A., Campo, S., Ferlazzo, A.M., Altavilla, D., and Calatroni, A. 2003. Efficacy of treatment with glycosaminoglycans on experimental collagen‐induced arthritis in rats. Arthritis Res. Ther. 5:R122‐R131.
   Cook, A.D., Rowley, M.J., Stockman, A., Muirden, K.D., and Mackay, I.R. 1994. Specificity of antibodies to type II collagen in early rheumatoid arthritis. J. Rheumatol. 21:1186‐1191.
   Cuzzocrea, S., McDonald, M.C., Mota‐Filipe, H., Mazzon, E., Costantino, G., Britti, D., Mazzullo, G., Caputi, A.P., and Thiemermann, C. 2000. Beneficial effects of tempol, a membrane‐permeable radical scavenger, in a rodent model of collagen‐induced arthritis. Arthritis Rheum. 43:320‐328.
   Henderson, B., Staines, N.A., Burrai, I., and Cox, J.H. 1984. The anti‐arthritic and immunosuppressive effects of cyclosporine on arthritis induced in the rat by type II collagen. Clin. Exp. Immunol. 57:51‐56.
   Magari, K., Nishigaki, F., Sasakawa, T., Ogawa, T., Miyata, S., Ohkubo, Y., Mutoh, S., and Goto, T. 2003. Anti‐arthritic properties of FK506 on collagen‐induced arthritis in rats. Inflamm. Res. 52:524‐529.
   Probert, A.W. Jr., Schrier, D.J., and Gilbertsen, R.B. 1984 Effects of antiarthritic compounds on type II collagen‐induced arthritis in rats. Arch. Int. Pharmacodyn. Ther. 269:167‐176.
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