Overview of Electrophysiological Techniques

Alan D. Wickenden1

1 Janssen R&D, San Diego, California
Publication Name:  Current Protocols in Pharmacology
Unit Number:  Unit 11.1
DOI:  10.1002/0471141755.ph1101s64
Online Posting Date:  March, 2014
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This unit provides an overview of the principal electrophysiological techniques commonly used for the study of ionic currents and the ion channels that mediate them. These techniques include electroencephalograms (EEGs), electrocardiograms (ECGs), single‐ and multiunit extracellular recording, multielectrode arrays, transepithelial recording, impedance measurements, and current‐clamp, voltage‐clamp, patch‐clamp, and lipid bilayer recording. The unit also discusses recent advances in high‐throughput, automated electrophysiological techniques for drug discovery and the use of stem cells as a tissue source. Curr. Protoc. Pharmacol. 64:11.1.1‐11.1.17. © 2014 by John Wiley & Sons, Inc.

Keywords: extracellular recording; intracellular recording; patch clamp; planar patch clamp; drug discovery; stem cells

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Table of Contents

  • Introduction
  • Electrophysiological Techniques
  • Electrophysiology and Drug Discovery
  • Tissue Sources for Electrophysiology
  • Outsourcing
  • Acknowledgements
  • Literature Cited
  • Figures
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Literature Cited

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