Overview of Electrophysiological Characterization of Neuronal Nicotinic Acetylcholine Receptors

Sonia Bertrand1, Daniel Bertrand1

1 Centre Médical Universitaire, Geneva, Switzerland
Publication Name:  Current Protocols in Pharmacology
Unit Number:  Unit 11.7
DOI:  10.1002/0471141755.ph1107s23
Online Posting Date:  February, 2004
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Abstract

Electrophysiology is one of the best tools to characterize ligand‐gated channels such as neuronal nicotinic acetylcholine receptors. In this unit, the properties of these receptors are discussed along with approaches for how they can be characterized. Special emphasis is given to agonist and antagonist profiles as well as allosteric effectors that offer alternative possibilities in drug discovery.

Keywords: neuronal nicotinic acetylcholine receptors; ligand‐gated channels; electrophysiology; characterization; agonist; antagonist; allosteric modulation

     
 
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Table of Contents

  • Introduction
  • Drug Application Methods and Considerations
  • Voltage Clamp Versus Current Clamp
  • Receptor Activation
  • Inhibition
  • Ionic Selectivity
  • Desensitization
  • Allosteric Effectors
  • Upregulation
  • Receptors in Slices
  • Technical Conditions for Specific Systems
  • Literature Cited
  • Figures
     
 
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Materials

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Literature Cited

Literature Cited
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