Single‐Channel Analysis of Glutamate Receptors

Chris Shelley1

1 Present address: Department of Biology, Franklin and Marshall College, Lancaster
Publication Name:  Current Protocols in Pharmacology
Unit Number:  Unit 11.17
DOI:  10.1002/0471141755.ph1117s68
Online Posting Date:  March, 2015
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Abstract

This is a companion to UNIT 11.16: Single‐Channel Recording of Glutamate Receptors. Described here are techniques for analyzing single‐channel currents recorded from glutamate receptors to characterize their properties. In addition, issues that need to be taken into account when analyzing glutamate receptor single‐channel recording results are discussed. © 2015 by John Wiley & Sons, Inc.

Keywords: single channel; glutamate receptor; ion channel

     
 
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Table of Contents

  • Introduction
  • Data Formats
  • Single‐Channel Analysis
  • Basic Protocol 1: First Pass Analysis of Glutamate Receptor Single‐Channel Recordings
  • Basic Protocol 2: Analysis of Glutamate Receptor Single‐Channel Recordings
  • Alternate Protocol 1: Analysis of Glutamate Receptor Single‐Channel Recordings Using Noise Analysis
  • Basic Protocol 3: Analysis of Glutamate Receptor Sublevels
  • Basic Protocol 4: Fitting Single‐Channel Data to Kinetic Models
  • Commentary
  • Literature Cited
  • Figures
  • Tables
     
 
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Materials

Basic Protocol 1: First Pass Analysis of Glutamate Receptor Single‐Channel Recordings

  Materials
  • Single‐channel data
  • Computer
  • Analysis software (See Table 11.17.1)

Basic Protocol 2: Analysis of Glutamate Receptor Single‐Channel Recordings

  Materials
  • Single‐channel data
  • Computer
  • Analysis software (See Table 11.17.1)

Alternate Protocol 1: Analysis of Glutamate Receptor Single‐Channel Recordings Using Noise Analysis

  Materials
  • Single‐channel or macroscopic current data
  • Computer
  • Analysis software (See Table 11.17.1)

Basic Protocol 3: Analysis of Glutamate Receptor Sublevels

  Materials
  • Single‐channel data
  • Computer
  • Analysis software (See Table 11.17.1)

Basic Protocol 4: Fitting Single‐Channel Data to Kinetic Models

  Materials
  • Single‐channel or macroscopic current data
  • Computer
  • Analysis software (see Table 11.17.1)
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Figures

Videos

Literature Cited

Literature Cited
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