Metastatic Model of Colon Carcinoma in Mice: Utility in the Study of Tumor Growth and Progression

Susan Jones‐Bolin1, Bruce Ruggeri1

1 Cephalon, Inc., West Chester, Pennsylvania
Publication Name:  Current Protocols in Pharmacology
Unit Number:  Unit 14.5
DOI:  10.1002/0471141755.ph1405s38
Online Posting Date:  September, 2007
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Abstract

Colorectal cancer is the second leading cause of cancer deaths in the United States with an estimated 150,000 diagnosed cases and over 56,000 fatalities annually (Jemal et al., 2006). Approximately one‐third to one‐half of cases are localized to the colon and rectum and have a favorable prognosis, while one‐third to one‐half present with regional lymph node metastases at diagnosis and generally are refractory to various chemotherapeutic regimens. Treatment options (surgery, radiation, and chemotherapy) are limited and the disease carries a grave prognosis for many patients. An orthotopic model of colon carcinoma in mice provides a way to evaluate the pathogenesis of tumor growth and metastasis as an aid in developing effective therapies and to better understand the underlying biology of colon tumor growth and metastasis. The protocol described in this unit details the development and characterization of an orthotopic model of murine colon carcinoma in BALB/c mice with diffuse lymphatic and hepatic metastatic spread, closely mimicking the course of the human disease. Curr. Protoc. Pharmacol. 38:14.5.1‐14.5.13. © 2007 by John Wiley & Sons, Inc.

Keywords: colon carcinoma; athymic nude; BALB/c mice; orthotopic; metastasis

     
 
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Table of Contents

  • Commentary
  • Literature Cited
  • Figures
  • Tables
     
 
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Materials

Basic Protocol 1:

  Materials
  • CT‐26 murine colon carcinoma cell line obtained under a Materials Transfer Agreement (MTA) from Dr. James L. Abbruzzese, University of Texas (M. D. Anderson Cancer Center) or human colon carcinoma cell lines (such as HT‐29 or HCT‐116) from ATCC
  • Media for cell growth. This includes RPMI 1640 for the CT‐26 cell line, and MEM or McCoys 5a for the HT‐29 or HC‐116 cell lines (Gibco BRL)
  • 10% fetal bovine serum (FBS; Hyclone or ATCC)
  • Penicillin/streptomycin
  • Trypsin
  • 1× PBS
  • 6‐ to 8‐week‐old female (20 to 25 g) BALB/c mice (Charles River Labs) for the CT‐26 murine colon tumor cell line
  • 6‐ to 8‐week‐old female (20 to 25 g) athymic nude mice (Charles River Labs) for the human colon carcinoma cell lines (HT‐29 or HCT‐116)
  • Ketamine (100 mg/ml, 10 ml vial; Hanna Pharmaceutical Supply)
  • Xylazine (20 mg/ml, 20 ml vial; Webster Veterinary Supply)
  • Isoflurane
  • Surgical scrub (e.g., Nolvassan or betadine)
  • Saline: 0.9% (w/v) NaCl (sterile; VWR)
  • 5% CO 2 incubator
  • 50‐ml conical‐shaped centrifuge tube
  • Circulating water heating pad. (e.g., Gaymar T‐pump or similar product).
  • Sterile towels
  • Nose cone apparatus
  • Petri dishes, 70‐mm or larger diameter
  • Sterile gauze
  • Cordless rechargeable electric clippers (e.g., Wahl, Webster Veterinary Supply)
  • Sterile alcohol prep pads, medium (Nice Pak or Curity; VWR or Fisher Scientific)
  • Autoclave for sterilizing instruments, gauze sponges, wound clips
  • Two sets of surgical instruments including (VWR or Fine Science Tools):
    • 4.5‐inch Iris scissors, straight, sharp ends, sterile
    • 4.5‐inch tissue forceps, 1 × 2 teeth
    • 4.75‐inch Adson forceps or delicate dressing forceps, serrated
    • Suture: Vicryl 5/0 or 6/0 or PDS II 5/0, for closing peritoneal layer; one pack per five to ten mice (Webster Veterinary Supply)
    • 9‐mm wound clips, with applier and remover (VWR)
    • Disposable scalpels
    • 5.5‐inch Mayo‐Hegar or similar needle holder
  • Sterile gauze sponges (2 × 2 in.)
  • 27‐G needle
  • Ear punch or tattooing device to identify individual mice
  • Additional reagents and equipment for using a hemacytometer for cell counting (Phelan, 2006) and euthanasia by CO 2 asphyxiation (Donovan and Brown, )
NOTE: Ketamine is a CIII controlled substance, so a DEA license is needed to order it.
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Figures

Videos

Literature Cited

Literature Cited
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