Overview of Transgenic Glioblastoma and Oligoastrocytoma CNS Models and Their Utility in Drug Discovery

Fuyi Chen1, Albert Becker2, Joseph LoTurco3

1 Current address: Department of Neurology, Yale School of Medicine, New Haven, Conn., 2 Department of Neuropathology, University of Bonn Medical Center, Bonn, 3 Department of Physiology and Neurobiology, University of Connecticut, Storrs, Conn.
Publication Name:  Current Protocols in Pharmacology
Unit Number:  Unit 14.37
DOI:  10.1002/0471141755.ph1437s72
Online Posting Date:  March, 2016
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Many animal models have been developed to investigate the sources of central nervous system (CNS) tumor heterogeneity. Reviewed in this unit is a recently developed CNS tumor model using the piggyBac transposon system delivered by in utero electroporation, in which sources of tumor heterogeneity can be conveniently studied. Their applications for studying CNS tumors and drug discovery are also reviewed. © 2016 by John Wiley & Sons, Inc.

Keywords: central nervous system; tumor heterogeneity; piggyBac transposon; in utero electroporation; glioblastoma multiforme; anaplastic oligoastrocytoma; atypical teratoid rhabdoid tumor

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Table of Contents

  • Introduction
  • Somatic Transgenesis in Cerebral Cortex with In Utero Electroporation of piggyBac Transposon System
  • piggyBac IUE Approach‐Induced CNS Tumors
  • Applications of the piggyBac IUE CNS Tumor Model
  • In Vivo Tumor Imaging
  • Limitations and Solutions
  • Conclusion
  • Literature Cited
  • Figures
  • Tables
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Literature Cited

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