Guidelines for the Care and Use of Laboratory Animals in Biomedical Research

Susan Jones‐Bolin1

1 null, Thorndale, Pennsylvania
Publication Name:  Current Protocols in Pharmacology
Unit Number:  Appendix 4B
DOI:  10.1002/0471141755.pha04bs59
Online Posting Date:  December, 2012
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Abstract

This unit provides a general overview on topics related to the practical care and use of laboratory animals in biomedical research. These topics are briefly described and provide Web sites and/or research articles that can be accessed for more detailed information. While the primary focus is on the care and use of rats and mice bred for biomedical research, many of the Web sites listed provide information on other species used for this purpose. Curr. Protoc. Pharmacol. 59:A.4B.1‐A.4B.9. © 2012 by John Wiley & Sons, Inc.

Keywords: Animal Welfare Act; Public Health Service (PHS) Policy; AAALAC; IACUC; ILAR; guide; OLAW (Office of Laboratory Animal Welfare); animal housing and enrichment; blood sampling; compound administration; animal numbers and group size; pain recognition; anesthesia; analgesia; euthanasia

     
 
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Table of Contents

  • Animal Welfare
  • Animal Welfare Act
  • Institutional Animal Care and Use Committee (IACUC)
  • Literature Cited
  • Tables
     
 
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Materials

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Figures

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Literature Cited

Literature Cited
   Artwohl, J., Brown, P., Corning, B., and Stein, S. (ACLAM Task Force). 2006. Report of the ACLAM task force on rodent euthanasia. JAALAS 45:98‐105.
   Battaglia, G., Busceti, C.L., Molinaro, G., Biagioni, F., Traficante, A., Nicoletti, F., and Bruno, V. 2006. Pharmacological activation of mGlu4 metabotropic glutamate receptors reduces nigrostriatal degeneration in mice treated with 1‐methyl‐4‐phenyl‐1,2,3,6‐tetrahydropyridine. J. Neurosci. 26:7222‐7229.
   Bayne, K.A. 2005. Potential for unintended consequences of environmental enrichment for laboratory animals and research results. ILAR J. 46:129‐139.
   Bruce, R.D. 1985. An up‐and‐down procedure for acute toxicity testing. Fund. Appl. Toxicol. 5:151‐157.
   Castro, C.A., Hogan, J.B., Benson, K.A., Shehata, C.W., and Landauer, M.R. 1995. Behavioral effects of vehicles: DMSO, ethanol, Tween‐20, Tween‐80, and emulphor‐620. Pharmacol. Biochem. Behav. 50:521‐526.
   Conlee, K.M., Stephens, M.L., Rowan, A.N., and King, L.A. 2005. Carbon dioxide for euthanasia: Concerns regarding pain and distress, with special reference to mice and rats. Lab. Anim. 39:137‐161.
   Danneman, P.J., Stein, S., and Walshaw, S.O. 1997. Humane and practical implications of using carbon dioxide mixed with oxygen for anesthesia or euthanasia of rats. Lab. Anim. Sci. 47:376‐385.
   Davis, J.A. 2008. Mouse and rat anesthesia and analgesia. Curr. Protoc. Neurosci. 42:A.4B.1‐A.4B.21.
   Diehl, K‐H., Hull, R., Morton, D., Pfister, R., Rabemampianina, Y., Smith, D., Vidal, J‐M., and Van De Vorstenbosch, C. 2001. A good practice guide to the administration of substances and removal of blood, including routes and volumes. J. Appl. Toxicol. 21:15‐23.
   Eskola, S., Lauhikari, M., Voipio, H.M., Laitinen, M., and Nevalainen, T. 1999. Environmental enrichment may alter the number of rats needed to achieve statistical significance. Scand. J. Lab. Anim. Sci. 26:134‐144.
   Festing, M.F. 1992. The scope for improving the design of laboratory animal experiments. Lab. Anim. 26:256‐268.
   Festing, M.F. and Altman, D.G. 2002. Guidelines for the design and statistical analysis of experiments using laboratory animals. ILAR J. 43:244‐258.
   Fish, R.E., Brown, M.J., Danneman, P.J., and Karas, A.Z. 2008. Anesthesia and Analgesia in Laboratory Animals. 2nd ed., Elsevier, Philadelphia, Penna.
   Flecknell, P.A. 1996a. Anaesthesia and analgesia for rodents and rabbits. In Handbook of Rodent and Rabbit Medicine (K. Laber‐Laird, M.M. Swindle, and P.A. Flecknell, eds.) pp. 219‐237. Pergammon Press, Butterworth‐Heineman, Newton, Mass.
   Flecknell, P.A. 1996b. Laboratory Animal Anaesthesia: An Introduction for Research Workers and Technicians, 2nd ed. Academic Press, London, U.K.
   Gaines Das, R.E. 2002. Role of ancillary variables in the design, analysis, and interpretation of animal experiments. ILAR J. 43:214‐222.
   Gärtner, K. 1999. Cage enrichment occasionally increases deviation of quantitative traits. In Proc. Int. Joint Mtg 12th ICLAS General Assembly and Conference and 7th FELASA Symposium. pp. 207‐210.
   Hackbarth, H., Kuppers, N., and Bohnet, W. 2000. Euthanasia of rats with carbon dioxide: Animal welfare aspects. Lab. Anim. 34:91‐96.
   Kirkden, R.D., Niel, L., Stewart, S.A., and Weary, D.M. 2008. Gas killing of rats: The effect of supplemental oxygen on aversion to carbon dioxide. Anim. Welf. 17:79‐87.
   Klaunberg, B.A., O'Malley, J., Clark, T., and Davis, J.A. 2004. Euthanasia of mouse fetuses and neonates. Contemp. Top. Lab. Anim. Sci. 43:29‐34.
   Leach, M.C., Bowell, V.A., Allan, T.F., and Morton, D.B. 2002. Aversion to gaseous euthanasia agents in rats and mice. Comp. Med. 52:249‐257.
   Liles, J.H. and Flecknell, P.A. 1992a. The effects of buprenorphine, nalbuphine and butorphanol alone or following halothane anaesthesia in food and water consumption and locomotor movement in rats. Lab. Anim. 26:180‐189.
   Liles, J.H. and Flecknell, P.A. 1992b. The use of non‐steroidal anti‐inflammatory drugs for the relief of pain in laboratory rodents and rabbits. Lab. Anim. 26:242‐255.
   Meyer, S.A., Marchand, A.J., Hight, J.L., Roberts, G.H., Escalon, L.B., Inouye, L.S., and MacMillan, D.K. 2005. Up‐and‐down procedure (UDP) determinations of acute oral toxicity of nitroso degradation products of hexahydro‐1,3,5‐trinitro‐1,3,5‐triazine (RDX). J. Appl. Toxicol. 25:427‐434.
   Mortom, D.B., Jennings, M., Beckwell, A., Ewbank, R., Godfrey, C., Holgayte, B., Inglis, I., James, R., Page, C., Sharman, I., Verschoyle, R., Westall, L., and Wilson, A.B. 2001. Refining procedures for the administration of substances. Report of the BVAAWF/FRAME/RSPCA/UFAW joint working group on refinement. Lab. Anim. 35:1‐41.
   Newberry, R.C. 1995. Environmental enrichment: Increasing the biological relevance of captive environments. Appl. Anim. Behav. Sci. 44:229‐243.
   Niel, L., Stewart, S.A., and Weary, D.M. 2008. Effect of flow rate on aversion to gradual fill carbon dioxide exposure in rats. Appl. Anim. Behav. Sci. 109:77‐84.
   Pritchett‐Corning, K.R. 2009. Euthanasia of neonatal rats with carbon dioxide. JAALAS 48:23‐27.
   Tsai, P.P., Stelzer, H.D., Hedrich, H.J., and Hackbarth, H. 2003. Are the effects of different enrichment designs on the physiology and behaviour of DBA/2 mice consistent? Lab. Anim. 37:314‐327.
   Vogel, V., Sanchez, C., and Jennum, P. 2002. EEG measurements by means of radiotelemetry after intracerebroventricular (ICV) cannulation in rodents. J. Neurosci. Methods 118:89‐96.
   Wolfer, D.P., Litvin, O., Morf, S., Nitsch, R.M., Lipp, H.P., and Würbel, H. 2004. Laboratory animal welfare: Cage enrichment and mouse behaviour. Nature 432:821‐822.
   Zhang, R.D., Price, J.E., Fujimaki, T., Bucana, C.D., and Fidler, I.J. 1992. Differential permeability of the blood‐brain barrier in experimental brain metastases produced by human neoplasms implanted into nude mice. Am. J. Pathol. 141:1115‐1124.
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