Overview of Protein Purification and Characterization

R.K. Scopes1

1 La Trobe University, Bundoora
Publication Name:  Current Protocols in Protein Science
Unit Number:  Unit 1.1
DOI:  10.1002/0471140864.ps0101s00
Online Posting Date:  May, 2001
GO TO THE FULL TEXT: PDF or HTML at Wiley Online Library

Abstract

This insightful overview by one of the most respected names in protein research discusses a broad array of the aspects involved with protein purification, including historical background, determining the purpose for purifying a particular protein, and actual methods and recommendations for purification procedures. Also covered are methods of analysis and detection, and techniques for characterizing a purified protein. Finally, a useful discussion of the equipment needed in a protein purification lab is provided.

     
 
GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online Library

Table of Contents

  • Aims and Objectives
  • Sources of Material for Protein Purification
  • Detection and Assay of Proteins
  • Methods for Separation and Purification of Proteins
  • Characterization of the Protein Product
  • The Protein Purification Laboratory
     
 
GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online Library

Materials

GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online Library

Figures

Videos

Literature Cited

Literature Cited
   Cohn, E.J., Strong, L.E., Hughes, W.L., Mulford, D.J., Ashworth, J.N., Melin, M. and Taylor, H.L. 1946. Preparation and properties of serum and plasma proteins. IV. A system for the separation into fractions of the proteins and lipoprotein components of biological tissues and fluids. J. Am. Chem. Soc. 68:459‐475.
Key References
   Deutscher, M.P., (ed.) 1990. Guide to protein purification. Methods Enzymol. 182:1‐894.
  Extensive collection of purification methods with some general protocols and examples.
   Janson, J.‐C. and Ryden, L.G. 1989. Protein Purification: Principles, High Resolution Methods, and Applications. VCH Publishers, New York.
  A useful collection of methods and examples.
  Kennedy, J.F. and Cabra, J.M., (eds.) 1993. Recovery Processes for Biological Materials. John Wiley & Sons, New York.
  A useful introduction to the problems of large‐scale methods.
   Kenny, A. and Fowell, S., (ed.) 1992. Practical protein chromatography. Methods Mol. Biol. 11:1‐327.
  Extensive descriptions of affinity chromatographic techniques with protocols and recipes.
   Scopes, R.K. 1993. Protein Purification. Principles and Practice, 3rd ed. Springer‐Verlag, New York and Heidelberg.
  General principles of all the main techniques used in purifying proteins. A useful laboratory handbook; does not include recipes or procedures for specific proteins.
GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online Library